Probiotics alter biofilm formation and the transcription ofPorphyromonas gingivalisvirulence-associated genes
Probiotics alter biofilm formation and the transcription ofPorphyromonas gingivalisvirulence-associated genes
复制标题
DOI：
10.1080/20002297.2020.1805553
复制
发表时间：
2020-01-01
影响因子：
4.5
通讯作者：
Alves Mayer, Marcia Pinto
中科院分区：
文献类型：
--
作者：
Ishikawa, Karin Hitomi;Mita, Daniela;Alves Mayer, Marcia Pinto
背景与目的益生菌在预防和控制牙周炎和其他慢性炎症方面的潜力已被提出。LactobacillusandBifidobacteriumspecies influenceP。牙龈素与牙龈上皮细胞（GECs）相互作用，但可能不是以独特的方式起作用。以选择最合适的抗stp益生菌。我们旨在评估几种菌株对牙龈卟啉单胞菌生物膜形成和转录毒力相关因子（PgVAFs）的影响。方法无细胞pH中和上清液（CFS）和活乳杆菌。andBifidobacteriumspp。对stp进行了测试。在单种和多种口腔链球菌中，atcc 33277和W83。gordonii)生物膜。p的相对转录。在生物膜和GECs共感染下检测牙龈毒性基因（fimA, mfa1, kgp, rgp, ftsHandluxS）。结果益生菌CFS可降低p。单物种生物膜和活益生菌中的atcc 33277水平降低p。牙龈素在多物种生物膜中的丰度。acidophilusLA5下调了生物膜和gec中大多数PgVAFs的转录。结论益生菌对tp有影响。通过下调整体PgVAFs来形成牙龈生物膜，其中对l的影响最为明显。acidophilusLA5。
Background and Objective The potential of probiotics on the prevention and control of periodontitis and other chronic inflammatory conditions has been suggested.LactobacillusandBifidobacteriumspecies influenceP. gingivalisinteraction with gingival epithelial cells (GECs) but may not act in a unique way. In order to select the most appropriate probiotic againstP. gingivalis, we aimed to evaluate the effect of several strains onPorphyromonas gingivalisbiofilm formation and transcription virulence-associated factors (PgVAFs). Methods Cell-free pH neutralized supernatants (CFS) and livingLactobacillusspp. andBifidobacteriumspp. were tested againstP. gingivalisATCC 33277 and W83, in mono- and multi-species (withStreptococcus oralisandS. gordonii) biofilms. Relative transcription ofP. gingivalisgenes (fimA, mfa1, kgp, rgp, ftsHandluxS) was determined in biofilms and under GECs co-infection. Results Probiotics CFS reducedP. gingivalisATCC 33277 levels in mono-species biofilms and living probiotics reducedP. gingivalisabundance in multi-species biofilms.L. acidophilusLA5 down-regulated transcription of most PgVAFs in biofilms and GECs. Conclusions Probiotics affectP. gingivalisbiofilm formation by down-regulating overall PgVAFs with the most pronounced effect observed forL. acidophilusLA5.