UK Biobank pilot study: Stability of haematological and clinical chemistry analytes
UK Biobank pilot study: Stability of haematological and clinical chemistry analytes
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DOI：
10.1093/ije/dym280
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发表时间：
2008-04-01
影响因子：
7.7
通讯作者：
Elliott, Paul
中科院分区：
文献类型：
--
作者：
Jackson, Chris;Best, Nicky;Elliott, Paul
血液和尿液中的分析物可能随着时间的推移而变化，这取决于运输和储存的条件。方法在英国生物样本库（Biobank）对40例患者的42种血液及临床化学分析物和4种尿液分析物进行时间稳定性研究。随机效应线性回归用于模拟样品重复检测结果随时间的变化，允许个体之间的异质性和检测变异性。结果随着时间的推移，大多数分析物的分析结果倾向于显示一个小的负偏差（1-3% / 12小时）。在几乎所有分析物中，个体间时间趋势的差异具有统计学意义（P < 0.05），主要是基线（时间0）测定结果的差异，可能除了平均红细胞血红蛋白浓度（MCHC）。46项分析中有4项（血清钙、胆固醇、纤维蛋白原和高密度脂蛋白胆固醇）预测未来个体呈阴性时间趋势的概率为>.9。冻融样品的结果与非冻融样品的结果没有实质性差异，除了分析结果的稳定性仅在12小时内进行评估。结论结果表明，与个体差异和分析误差相比，检测结果在36小时内的任何不稳定性都可能很小。在0到36小时之间的任何时间进行的单次分析测量都应该给出该个体在0时间的分析物浓度的代表性值。
Background Analytes in blood and urine may vary over time according to conditions of transport and storage.Methods UK Biobank pilot study to investigate stability through time of 42 haematological and clinical chemistry analytes in blood and four analytes in urine, kept in storage for up to 36h, for 40 individuals. Random effects linear regressions were used to model the change through time in repeated assay results on a sample, allowing for heterogeneity between individuals and assay variability. Results Assay results for most analytes tended to show a small negative bias (1-3% per 12 h stored) over time on average. Statistically significant (P < 0.05) heterogeneity in time trends between individuals, found for nearly all analytes, was dominated by differences in the baseline (time 0) assay results with the possible exception of Mean Corpuscular Haemoglobin Concentration (MCHC). Four out of 46 analytes (serum calcium, cholesterol, fibrinogen and HDL cholesterol) had a predicted probability of a negative time trend for a future individual > 0.9.Results for freeze-thaw samples were not materially different from those for non-freeze-thaw samples, except that stability of the analyte results was only assessed up to 12 h.Conclusions The results suggest that any instability in assay results up to 36h is likely to be small in comparison with between individual differences and assay error, and that a single assay measurement at any time between 0 and 36h should give a representative value of the analyte concentration at time zero for that individual.