Fluorescence lifetime in cardiovascular diagnostics
Fluorescence lifetime in cardiovascular diagnostics
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DOI：
10.1117/1.3327279
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发表时间：
2010-01-01
影响因子：
3.5
通讯作者：
Marcu, Laura
中科院分区：
文献类型：
--
作者：
Marcu, Laura
我们回顾了荧光寿命技术，包括时间分辨激光诱导荧光光谱 (TR-LIFS) 和荧光寿命成像显微镜 (FLIM) 仪器以及可用于表征和诊断动脉粥样硬化斑块的相关方法。重点是 TR-LIFS 和 FLIM 的转化研究潜力，以及确定内在荧光信号是否可用于为高风险动脉粥样硬化斑块的诊断提供有用的对比。我们的结果表明，这些技术可以区分与动脉粥样硬化斑块不稳定和破裂有关的重要生化特征，并显示出它们在未来血管内应用的潜力。 (C) 2010 年光电仪器工程师协会。 [DOI：10.1117/1.3327279]
We review fluorescence lifetime techniques including timeresolved laser-induced fluorescence spectroscopy (TR-LIFS) and fluorescence lifetime imaging microscopy (FLIM) instrumentation and associated methodologies that allow for characterization and diagnosis of atherosclerotic plaques. Emphasis is placed on the translational research potential of TR-LIFS and FLIM and on determining whether intrinsic fluorescence signals can be used to provide useful contrast for the diagnosis of high-risk atherosclerotic plaque. Our results demonstrate that these techniques allow for the discrimination of important biochemical features involved in atherosclerotic plaque instability and rupture and show their potential for future intravascular applications. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3327279]