Tapers and restrictors for capillary electrochromatography and capillary electrochromatography mass spectrometry
Tapers and restrictors for capillary electrochromatography and capillary electrochromatography mass spectrometry
复制标题
DOI：
10.1016/s0021-9673(96)01081-3
复制
发表时间：
1997-04-18
影响因子：
4.1
通讯作者：
King, BW
中科院分区：
文献类型：
--
作者：
Lord, GA;Gordon, DB;King, BW
锥形和窄限流器毛细管已被研究用于毛细管电色谱（CEC），并已被证明提供可行的，更可重复的，替代二氧化硅玻璃料，并可能有助于抑制气泡的形成，在CEC中公认的问题。此外，这些毛细管已被耦合到质谱，特别是低流量和纳米流量电喷雾电离，其中的结果表明，更好地保持色谱效率和更高的灵敏度，与更传统的接口相比。建议使用锥形CEC柱入口，用于从微环境（如细胞内容物）中取样。
Tapered and narrow restrictor capillaries have been investigated for use in capillary electrochromatography (CEC) and have been shown to offer viable, more reproducible, alternatives to silica frits and may aid in suppression of bubble formation, a recognised problem in CEC. In addition, these capillaries have been coupled to mass spectrometry, especially for low-flow and nano-flow electrospray ionisation, where results have shown better maintenance of chromatographic efficiency and higher sensitivity, compared with more conventional interfaces. The use of tapered CEC column inlets is suggested as being useful in sampling from micro-environments, such as cell contents.