Combination of morphological feature analysis and immunohistochemistry is useful for screening of EML4-ALK-positive lung adenocarcinoma
Combination of morphological feature analysis and immunohistochemistry is useful for screening of EML4-ALK-positive lung adenocarcinoma
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DOI：
10.1136/jcp.2010.081166
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发表时间：
2010-12-01
影响因子：
3.4
通讯作者：
Tsujimoto, Masahiko
中科院分区：
文献类型：
--
作者：
Jokoji, Ryu;Yamasaki, Takashi;Tsujimoto, Masahiko
背景肺癌中含有棘皮微管相关蛋白样间变性淋巴瘤激酶(EML4-ALK)融合基因。近年来，免疫组织化学检测EML4-ALK阳性肺腺癌的敏感性几乎与荧光原位杂交(FISH)方法相当。目的研究大量手术切除标本中EML4-ALK阳性肺腺癌的临床病理特征，以建立一种实用的EML4-ALK阳性肺腺癌筛查方法。方法采用免疫组织化学方法对254例手术切除标本进行EML4-ALK阳性肺腺癌筛查。结果：肺腺癌中EML4-ALK阳性病例占3.1%(8/254)。EML4-ALK阳性的肺腺癌与胞浆内和/或胞外粘蛋白显著相关(p=0.0001)，筛状型与胞浆外粘蛋白过多有关(p<0.05)。
Background A subset of lung cancers harbours the fusion gene echinoderm microtubule-associated protein-like-4-anaplastic lymphoma kinase (EML4-ALK). Recently, immunohistochemistry for ALK has shown sensitivity for the detection of EML4-ALK-positive lung adenocarcinoma almost equal to that of the fluorescence in situ hybridisation (FISH) assay.Aims To study the clinicopathological features of EML4-ALK-positive lung adenocarcinoma in a large number of surgically resected samples using immunohistochemistry, in order to establish a useful screening method for EML4-ALK-positive lung adenocarcinoma.Methods Immunohistochemistry for ALK was used to screen for EML4-ALK-positive lung adenocarcinomas in 254 cases of surgically resected samples.Results EML4-ALK-positive cases were detected in 3.1% of lung adenocarcinomas (8/254). EML4-ALK-positive lung adenocarcinomas showed significant associations with intra- and/or extra-cytoplasmic mucin (p=0.0001), and cribriform pattern with excessive extracytoplasmic mucin (p