Effects of Low Molecular Weight Fucoidan on the Proliferation and Apoptosis of Dysplastic Oral Keratinocyte and Oral Squamous Cell Carcinoma Cells
Effects of Low Molecular Weight Fucoidan on the Proliferation and Apoptosis of Dysplastic Oral Keratinocyte and Oral Squamous Cell Carcinoma Cells
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低分子量岩藻多糖对发育不良性口腔角质形成细胞和口腔鳞状细胞癌细胞增殖和凋亡的影响
DOI：
10.1177/1934578x20921681
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发表时间：
2020-04-01
影响因子：
1.8
通讯作者：
Wang, Wanchun
中科院分区：
文献类型：
--
作者：
Li, Lulu;Xu, Yingjie;Wang, Wanchun
口腔鳞状细胞癌（Oral squamous cell carcinoma，OSCC）是口腔常见的恶性肿瘤之一，发病率高，进展快，死亡率高。目前口腔鳞癌的治疗方法仍存在一定的缺陷，严重影响了患者的生活质量。因此，寻找一种更安全、更有效的治疗口腔鳞癌的方法是当务之急。低分子量岩藻聚糖硫酸酯（LMWF）具有抗肿瘤、抗炎、抗血栓形成等多种生物活性，且无明显毒副作用，但其对口腔鳞癌的影响尚未见报道。本研究采用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四氮唑溴化物法、流式细胞术、TUNEL法、集落形成法和伤口愈合实验检测LMWF对口腔黏膜上皮细胞（DOK）和口腔鳞癌细胞（SCC-9和SCC-2,5）的影响，并采用RT-qPCR法检测Notch-1和Jagged-1的表达水平。结果表明，LMWF能抑制DOK和SCC-9细胞的增殖和迁移，促进细胞凋亡。低分子量褐藻糖胶上调DOK和SCC-9细胞中Notch-1和Jagged-1的表达。提示LMWF可能通过上调Notch信号通路促进DOK和SCC-9细胞凋亡，从而抑制细胞增殖，发挥抗肿瘤作用。为开发LMWF作为新型口腔癌治疗药物提供了理论依据。
Oral squamous cell carcinoma (OSCC) is one of the most common malignant tumors with high incidence, rapid progress, and high mortality. There are still some defects in the treatments of OSCC, which seriously affect the quality of patients' life. Therefore, it is urgent to find a safer and more effective treatment for OSCC. Low molecular weight fucoidan (LMWF) has various biological activities, such as antitumor, anti-inflammatory, and antithrombotic, and has no obvious side effects, but the effect of LMWF on OSCC has not been reported. In this study, the effects of LMWF on dysplastic oral keratinocyte (DOK) and OSCC cells SCC-9 and SCC-25 were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, TUNEL, colony formation, and wound healing assay, and the expression levels of Notch-1 and Jagged-1 treated with LMWF were detected by RT-qPCR. The results showed that LMWF could inhibit the proliferation and migration of DOK and SCC-9, and promote apoptosis. Low molecular weight fucoidan upregulated the expression of Notch-1 and Jagged-1 in DOK and SCC-9 cells. It indicated that LMWF might promote the apoptosis of DOK and SCC-9 by upregulating the Notch signal pathway, thereby inhibiting cell proliferation and playing an antitumor role. It provided theoretical basis to develop LMWF as a novel therapeutic drug for oral cancer.