Expression of E-cadherin cell adhesion molecules in human breast cancer tissues and its relationship to metastasis.
Expression of E-cadherin cell adhesion molecules in human breast cancer tissues and its relationship to metastasis.
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发表时间：
1993-04
期刊：
影响因子：
11.2
通讯作者：
H. Oka;H. Shiozaki;K. Kobayashi;M. Inoue;H. Tahara;Tetsurou Kobayashi;Y. Takatsuka;N. Matsuyoshi;S. Hirano;M. Takeichi;T. Mori
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作者：
H. Oka;H. Shiozaki;K. Kobayashi;M. Inoue;H. Tahara;Tetsurou Kobayashi;Y. Takatsuka;N. Matsuyoshi;S. Hirano;M. Takeichi;T. Mori
E-钙粘蛋白（E-cad）是钙粘蛋白家族的一个亚类，在维持上皮组织中的细胞间连接中起主要作用。为探讨E-cad表达与乳腺癌侵袭转移的关系，我们应用免疫组化方法检测了120例乳腺癌组织中E-cad的表达。在非癌性上皮细胞中，E-cad在细胞-细胞边界上强烈表达，而在肿瘤中观察到各种染色模式。其中56例（47%）呈Pr型表达，64例（53%）呈Rd型或阴性表达。我们发现E-cad表达与临床病理特征之间存在显著相关性。Rd型在浸润性导管癌（58%，56/97）和低分化癌（84%，21/25）中的频率显著高于非浸润性和高分化癌。此外，在以下晚期肿瘤中检测到高频率的Rd型：T3，4肿瘤，71%（31例中的22例）;具有广泛淋巴结转移的肿瘤，74%（39例中的29例）;以及具有远处转移的肿瘤，86%（22例中的19例）。这些值明显高于其对应物。E-cad阳性肿瘤中表皮生长因子受体表达呈阳性趋势。然而，E-cad表达，绝经状态，激素受体状态和DNA倍体模式之间没有显着的关系。提示E-cad表达降低可能在乳腺癌侵袭转移中起重要作用。
E-cadherin (E-cad) is a subclass of the cadherin family that plays a major role in maintenance of intercellular junctions in epithelial tissues. In order to explore the correlation between the expression of E-cad and cancer invasion and metastasis in vivo, we performed an immunohistochemical examination for E-cad expression in 120 patients with breast cancer using our specific anti-E-cad monoclonal antibody. In noncancerous epithelial cells, E-cad was strongly expressed on cell-cell boundaries, whereas various staining patterns were observed in tumors. Of these 120 tumors, 56 (47%) showed Pr type expression of E-cad, and 64 (53%) showed Rd type or negative expression. We found significant correlations between E-cad expression and clinicopathological features. The frequency of Rd type was significantly higher in invasive ductal carcinomas (58%, 56 of 97) and poorly differentiated carcinomas (84%, 21 of 25) than in noninvasive and well-differentiated carcinomas. Furthermore, a high frequency of Rd type was detected in the following advanced tumors: T3,4 tumors, 71% (22 of 31); tumors with extensive lymph node metastasis, 74% (29 of 39); and tumors with distant metastasis, 86% (19 of 22). These values were significantly higher compared with their counterparts. The expression of epidermal growth factor receptor tended to be positive in E-cad-positive tumors. However, no significant relationship was seen among E-cad expression, menopausal status, hormone receptor status, and DNA ploidy pattern. These results suggest that the reduction of E-cad expression may play an important role in invasion and metastasis of human breast cancer.