GC-MS determination of heroin metabolites in meconium: evaluation of four solid-phase extraction cartridges.
GC-MS determination of heroin metabolites in meconium: evaluation of four solid-phase extraction cartridges.
复制标题
GC-MS 测定胎粪中的海洛因代谢物：四种固相萃取柱的评估。
DOI：
10.1093/jat/25.2.93
复制
发表时间：
2001
影响因子：
2.5
通讯作者：
ElSohly,MA
中科院分区：
文献类型：
--
作者：
Salem,MY;Ross,SA;Murphy,TP;ElSohly,MA
A procedure for extraction of heroin and metabolites for gas chromatography-mass spectrometry (GC-MS) analysis of meconium specimens that would allow detection of these analytes at low levels was needed. Solid-phase extraction (SPE) cartridges were therefore evaluated for their effectiveness in sample preparation. Four different types of commercially available extraction cartridges were used. Heroin, 6-monoacetylmorphine (6-MAM), morphine, and codeine were extracted from meconium samples using these SPE cartridges and then simultaneously analyzed using GC-MS. In each case, the extraction efficiency, linearity range, limit of detection (LOD), limit of quantitation (LOQ), between-run precision, and within-run precision were determined. Although satisfactory results were obtained with the four different types of SPE cartridges, best overall performance was observed using Clean Screen®columns following the procedures outlined here. LODs as low as 20 ng/g for codeine, 10 ng/g for morphine, and 2.5 ng/g for 6-MAM were obtained, and LOQs as low as 20 ng/g for codeine, 10 ng/g for morphine, and 5 ng/g for 6-MAM were obtained. In all cases linearities were observed (r= > 0.99) for codeine, morphine, and 6-MAM over a wide concentration range (100–2000, 100–2000, and 5–100, respectively). At 50 ng/g codeine and morphine and 10 ng/g 6-MAM, the precision of analysis using these cartridges showed coefficients of variation ranging from 4.75% to 15.5%.
