Bark beetle controls epithelial morphogenesis by septate junction maturation in Drosophila
Bark beetle controls epithelial morphogenesis by septate junction maturation in Drosophila
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DOI：
10.1016/j.ydbio.2015.02.008
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发表时间：
2015-04-15
影响因子：
2.7
通讯作者：
Schuh, Reinhard
中科院分区：
文献类型：
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作者：
Hildebrandt, Anja;Pflanz, Ralf;Schuh, Reinhard
上皮组织以不同的成分分隔身体区室。脊椎动物中的紧密连接（TJ）和无脊椎动物中的分隔连接（SJS）控制这些隔室之间的分子的细胞旁流动。TJ和SIS的这种上皮屏障功能必须在细胞增殖和细胞运动期间的组织形态发生中稳定维持。在这里，我们表明，树皮甲虫（树皮），一个假定的跨膜清道夫受体样蛋白，是必不可少的成熟，但不是建立在果蝇的SJS。缺乏树皮的胚胎建立了功能性的SJS，但由于在随后的胚胎发育过程中形成了基本的隔膜，这些SJS变得无功能。此外，细胞粘附在侧细胞膜受损，SJS的核心蛋白复合物被错误定位，但似乎在这些胚胎中以其他方式正常形成。我们提出了一个模型，其中树皮作为一个支架蛋白，介导细胞粘附和安装SJ核心复合物在组织形态发生细胞重排。(C)2015 Elsevier Inc. All rights reserved.
Epithelial tissues separate body compartments with different compositions. Tight junctions (TJs) in vertebrates and septate junctions (SJs) in invertebrates control the paracellular flow of molecules between these compartments. This epithelial barrier function of TJs and Sis must be stably maintained in tissue morphogenesis during cell proliferation and cell movement. Here, we show that Bark beetle (Bark), a putative transmembrane scavenger receptor-like protein, is essential for the maturation but not the establishment of SJs in Drosophila. Embryos that lack bark establish functional SJs, but due to rudimentary septae formation during subsequent embryonic development, these become nonfunctional. Furthermore, cell adhesion is impaired at the lateral cell membrane and the core protein complexes of SJs are mis-localised, but appear to form otherwise normally in such embryos. We propose a model in which Bark acts as a scaffold protein that mediates cell adhesion and mounting of SJ core complexes during cell rearrangement in tissue morphogenesis. (C) 2015 Elsevier Inc. All rights reserved.