Maximizing CO2 biofixation and lipid productivity of oleaginous microalga Graesiella sp. WBG-1 via CO2-regulated pH in indoor and outdoor open reactors.
Maximizing CO2 biofixation and lipid productivity of oleaginous microalga Graesiella sp. WBG-1 via CO2-regulated pH in indoor and outdoor open reactors.
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DOI：
10.1016/j.scitotenv.2017.10.127
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发表时间：
2018-04
期刊：
影响因子：
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通讯作者：
Zhongjie Wang;Xiaobin Wen;Yan Xu;Yi Ding;Yahong Geng;Yeguang Li
中科院分区：
文献类型：
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作者：
Zhongjie Wang;Xiaobin Wen;Yan Xu;Yi Ding;Yahong Geng;Yeguang Li
二氧化碳(CO2)和pH是影响微藻生长和脂肪积累的两个相互依存的因素。然而，这两个因素的影响通常是分开研究的。利用烟道气等外源CO2调节微藻规模化培养过程中的pH值，为CO2生物固定和生物柴油生产提供了一种理想的手段。本研究对含油性微藻Graesiellasp的CO2生物固定和产脂作用进行了研究。在10个L循环养殖池和5个m~2开式回旋区反应器中，对外源15%CO_2(烟气浓度)调节的4个pH水平进行了研究。结果表明，pH为8.0~9.0是固定化和产脂的最适pH，在L−1天−1和2天−1分别达到最高的CO2固定率0.26 mg和18.9 mg/m−，脂肪含量分别为46.28%和32.38%，产脂率分别为64.8 mg/L−1天−1和3.14 mg/m−2天−1。本研究还提出了CO2利用率与开放式反应器pH的正相关关系，为微藻固定CO2的筛选提供了方向。本研究为大规模培养中利用微藻固定二氧化碳和产脂提供了一种很好的策略。
Carbon dioxide (CO2) and pH are two interdependent factors that greatly impact the growth and lipid accumulation of microalgae. However, the effects of these two factors are usually studied separately. The use of exogenous CO2, such as flue gas derived, to regulate pH in the large-scale cultivation of microalgae provides an ideal means for combining CO2biofixation and biodiesel production. In this study, the CO2biofixation and lipid production of oleaginous microalgaGraesiellasp. WBG1 was explored for four pH levels regulated by exogenous 15% CO2(flue gas concentration) in 10 L circular culture ponds and 5 m2open raceway reactors. Results revealed that pH 8.0–9.0 was the optimum pH for CO2fixation and lipid production, attaining the highest CO2fixation rates of 0.26 g L− 1day− 1and 18.9 g m− 2day− 1, respectively, lipid contents of 46.28% and 32.38%, and lipid productivities of 64.8 mg L− 1day− 1and 3.14 g m− 2day− 1. A positive correlation between CO2utilization efficiency and pH in open reactors was also suggested in this research, and thus provides direction for screening of CO2fixation by microalgae. The present study provides an excellent strategy for coupling CO2fixation and lipid production via microalgae in large-scale cultivation.