Decrease in the expression level of the gene encoding the putative Bombyx mori bidensovirus receptor during virus infection
Decrease in the expression level of the gene encoding the putative Bombyx mori bidensovirus receptor during virus infection
复制标题
DOI：
10.1007/s00705-018-4017-1
复制
发表时间：
2018-12-01
影响因子：
2.7
通讯作者：
Kadono-Okuda, Keiko
中科院分区：
文献类型：
--
作者：
Ito, Katsuhiko;Fujii, Takeshi;Kadono-Okuda, Keiko
家蚕双腺病毒（BmBDV）是一种仅在家蚕中肠柱状细胞内复制的病原体，可引起家蚕的致死性疾病。对BmBDV的抗性不依赖于病毒剂量，由单个基因nsd-2（抗性基因）决定。以前，我们通过使用B的定位克隆鉴定了nsd-2。mori基因组信息，并发现该基因编码一个推定的氨基酸转运蛋白，可能作为BmBDV的受体发挥作用。为了了解BmBDV与病毒受体之间的关系，我们对病毒感染后+（nsd-2）（nsd-2的等位基因;易感基因）的表达进行了分析。使用从未感染和病毒感染的蚕的中肠分离的总RNA进行的定量RT-PCR分析显示，在未感染的蚕中+（nsd-2）的表达水平没有变化，而在病毒感染的蚕中+（nsd-2）的表达水平急剧下降。此外，BmBDV来源的转录物和+（nsd-2）之间的表达模式的比较显示，+（nsd-2）的表达水平随着病毒来源的转录物的增加而降低。此外，对中肠中编码其他转运蛋白的26个基因的表达分析表明，在病毒感染后，其他3个基因的表达水平也类似于+（nsd-2）表达水平的降低而降低。因此，我们的研究结果表明，一些转运蛋白，包括+（nsd-2），受到BmBDV感染。
Bombyx mori bidensovirus (BmBDV) is a pathogen that replicates only in the midgut columnar cells of silkworms, causing fatal disease. Resistance to BmBDV, which does not depend on the viral dose, is determined by a single gene, nsd-2 (resistance gene). Previously, we identified nsd-2 by positional cloning using B. mori genome information and found that this gene encodes a putative amino acid transporter that may function as a receptor for BmBDV. In this study, to understand the relationship between BmBDV and the putative virus receptor, we performed expression analysis of +(nsd-2) (allele of nsd-2; susceptibility gene) after virus infection. Quantitative RT-PCR analysis using total RNA isolated from the midgut of an uninfected and a virus-infected silkworm revealed no change in the expression levels of +(nsd-2) in the uninfected silkworm, whereas the expression levels of +(nsd-2) drastically decreased in the virus-infected silkworm. Moreover, comparison of the expression pattern between the BmBDV-derived transcript and +(nsd-2) revealed that the expression level of +(nsd-2) decreased with an increase in the virus-derived transcript. In addition, expression analysis of 26 genes encoding other transporters in the midgut demonstrated that the expression levels of three other genes also decreased similarly to the decrease of the expression levels of +(nsd-2) after virus infection. Thus, our results suggest that some transporters, including +(nsd-2), are affected by BmBDV infection.