A versatile and high-yielding protocol for the preparation of genomic DNA from Capsicum spp. (pepper)
A versatile and high-yielding protocol for the preparation of genomic DNA from Capsicum spp. (pepper)
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DOI：
10.21273/hortsci.32.5.937
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发表时间：
1997-08
期刊：
影响因子：
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通讯作者：
J. Prince;Yiping Zhang;E. Radwanski;M. Kyle
中科院分区：
文献类型：
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作者：
J. Prince;Yiping Zhang;E. Radwanski;M. Kyle
从辣椒属植物中分离基因组 DNA。 （胡椒）基于核酸杂交和/或聚合酶链反应（PCR）的分析所需的质量和数量已被证明是困难的。我们在这里报告了一种能够从各种辣椒基因型中可靠地产生足够数量的优质辣椒基因组 DNA 的程序。
The isolation of genomic DNA from Capsicum spp. (pepper) of the quality and quantity required for analyses based on nucleic acid hybridization and/or the polymerase chain reaction (PCR) has proven difficult. We report here a procedure that reliably yields sufficient quantities of good-quality pepper genomic DNA from a variety of Capsicum genotypes.