Ultrastructural localization of S-antigen in retinal structures.
Ultrastructural localization of S-antigen in retinal structures.
复制标题
S 抗原在视网膜结构中的超微结构定位。
作者：
R. M. Broekhuyse;J. Leunissen;A. Verkley
S-抗原（视网膜可溶性、致色素变性、51 kDa蛋白质）的定位已被研究。在间接免疫荧光技术中，高度特异性的兔抗牛S-抗原抗血清主要与大鼠视网膜感光细胞的外节层反应，条件是通过光适应动物的灌注固定组织。使用相同的固定技术和蛋白A包被的金颗粒进行标记，通过免疫电子显微镜获得更详细的信息。S-抗原被发现主要是结合到杆外段质膜和光盘。鉴于S-抗原的功能，这种本地化的可能含义进行了讨论。
The localization of S-antigen, the soluble, uveitogenic, 51 kDa protein of the retina has been studied. Highly specific rabbit anti-bovine S-antigen antiserum reacted predominantly with the outer segment layer of the photoreceptor cells of rat retina in the indirect immunofluorescence technique, provided that the tissue was fixed by perfusion of the light adapted animal. More detailed information was obtained by immuno-electron microscopy using the same fixation technique and Protein A-coated gold particles for labeling. S-antigen was found to be predominantly bound to the rod outer segment plasma membranes and to the discs. The possible implication of this localization is discussed in view of the function of S-antigen.