Microsatellite markers from' the Chagas disease vector, Rhodnius prolixus (Hemiptera, Reduviidae), and their applicability to Rhodnius species
Microsatellite markers from' the Chagas disease vector, Rhodnius prolixus (Hemiptera, Reduviidae), and their applicability to Rhodnius species
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DOI：
10.1016/j.meegid.2008.01.006
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发表时间：
2008-05-01
影响因子：
3.2
通讯作者：
Solignac, M.
中科院分区：
文献类型：
--
作者：
Harry, M.;Roose, C. L.;Solignac, M.
从委内瑞拉、哥伦比亚和中美洲的主要南美锥虫病载体Rhodnius prolixus的部分基因组文库中分离并鉴定了10个微卫星。这些多态性分子标记可能在南美锥虫病控制倡议中特别有用。所分离的引物对具有更广泛的适用性，在所测试的10个Rhodnius种中的5个中，即R.阿提库斯河纳苏图斯河疏忽草R. neivai和R.粗壮。有趣的是，所有的基因座都在后者中扩增。这些标志物可能是感兴趣的，以跟踪从锥蝽林种群的人类住所的殖民化，以更好地确定流行病学的风险模式。(c)2008 Elsevier B.V.保留所有权利。
Ten microsatellites were isolated and characterized from a partial genomic library of Rhodnius prolixus, the principal Chagas disease vector in Venezuela, Colombia and Central America. These polymorphic molecular markers could be particularly useful in Chagas disease control initiatives. A wider applicability of the primer-pairs isolated was shown, from 6 to 10 loci being amplifiable in five out of the ten Rhodnius species tested, namely R. domesticus, R. nasutus, R. neglectus, R. neivai and R. robustus. Interestingly, all the loci were amplified in the latter. These markers may be of interest to trace the colonization of human dwellings from triatomine sylvatic populations in order to better define epidemiological risk patterns. (c) 2008 Elsevier B.V. All rights reserved.