The RNF146 E3 ubiquitin ligase is required for the control of Wnt signaling and body pattern formation in Xenopus
The RNF146 E3 ubiquitin ligase is required for the control of Wnt signaling and body pattern formation in Xenopus
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RNF146 E3 泛素连接酶是非洲爪蟾 Wnt 信号传导和身体模式形成的控制所必需的
DOI：
10.1016/j.mod.2017.08.001
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发表时间：
2017-10-01
影响因子：
2.6
通讯作者：
Tao, Qinghua
中科院分区：
文献类型：
--
作者：
Zhu, Xuechen;Xing, Rui;Tao, Qinghua
The RING finger protein Rnf146 encodes an E3 ubiquitin ligase capable of targeting poly-ADP-ribosylated substrates for proteasomal degradation. Rnf146 has been identified as a critical regulator of Axinl and thus of Wnt43-catenin signaling. However its physiological significance in vertebrate embryonic development remains to be demonstrated. In this study, we take advantages of early Xenopus embryos to demonstrate that Rnf146 is essential for embryonic pattern formation. Depletion of zygotic Rnf146 using a translation blocking morpholino oligo (MO) results in anteriorized development and increased expression the anterior marker gene Otx2, consistent the notion that Rnf146 is a positive regulator of Wnt/(3-catenin signaling through negatively regulating Axinl expression. This notion is further supported by examination of the role of maternal Rnf146 in the context of Spemann organizer formation and dorsal axis development. Depletion of maternal Rnf146 using an antisense oligodeoxynucleic acid (ODN) leads to ventralized development and diminished expression of organizer genes. Together, we have provided evidence for the first time that Rnf146 is a critical regulator of embryonic pattern formation in vertebrates. (C) 2017 Published by Elsevier Ireland Ltd.
