FUNCTIONAL ISOLATION AND CHARACTERIZATION OF HUMAN HEMATOPOIETIC STEM-CELLS
FUNCTIONAL ISOLATION AND CHARACTERIZATION OF HUMAN HEMATOPOIETIC STEM-CELLS
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DOI：
10.1126/science.7528940
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发表时间：
1995-01-06
期刊：
影响因子：
56.9
通讯作者：
SCADDEN, DT
中科院分区：
文献类型：
--
作者：
BERARDI, AC;WANG, AL;SCADDEN, DT
造血细胞分化的步骤以获得或丧失特定的表型特征为标志。对早期作用细胞因子Kit配体和白细胞介素-3有反应的人骨髓细胞被迫代谢死亡。剩余的亚组分代表1/10（5）的骨髓单核细胞，通过细胞周期分析确定为静止，并具有干细胞免疫表型。这些细胞高度富集长期培养起始细胞，能够形成次级集落，并产生髓样和淋巴样后代。因此，这种技术上简单的策略导致了具有造血干细胞特征的细胞的有效纯化。
Hematopoietic cells differentiate in steps marked by the acquisition or loss of specific phenotypic characteristics. Human bone marrow cells that were responsive to the early-acting cytokines Kit ligand and interleukin-3 were forced to a metabolic death. The subfraction remaining represented 1 in 10(5) bone marrow mononuclear cells, was determined to be quiescent by cell cycle analysis, and had a stem cell immunophenotype, The cells were highly enriched for long-term culture-initiating cells, were capable of secondary colony formation, and produced both myeloid and lymphoid progeny. Thus, this technically simple strategy led to the efficient purification of cells with characteristics of hematopoietic stem cells.