In vitro evaluation of borate-based bioactive glass scaffolds prepared by a polymer foam replication method
In vitro evaluation of borate-based bioactive glass scaffolds prepared by a polymer foam replication method
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DOI：
10.1016/j.msec.2009.05.013
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发表时间：
2009-08-31
影响因子：
7.9
通讯作者：
Liu, Xin
中科院分区：
文献类型：
--
作者：
Fu, Hailuo;Fu, Qiang;Liu, Xin
采用聚合物泡沫复制方法制备了微观结构与人小梁骨相似的硼酸盐基生物活性玻璃支架，并在体外评估其潜在的骨修复应用。支架（孔隙率=72+/-3%；孔径=250-500μm）的抗压强度为6.4+/-1.0MPa。通过在 37℃ 的 0.02 M K2HPO4 溶液中 7 天内在玻璃表面形成羟基磷灰石 (HA) 层来证实支架的生物活性。支架的生物相容性通过细胞对支架溶解产物提取物的反应进行评估，使用 MTT 水解、细胞活力和碱性磷酸酶活性测定。当硼浓度低于阈值（0.65 mM）时，玻璃溶解产物的提取物支持骨髓基质细胞的增殖，以及小鼠 MLO-A5 细胞（一种成骨细胞系）的增殖和功能。扫描电镜显示玻璃支架表面培养的MLO-A5细胞附着且密度持续增加。结果表明，只要玻璃中释放的硼可以控制在阈值以下，硼酸盐基生物活性玻璃可能成为骨组织工程的潜在支架材料。 (C) 2009 Elsevier B.V. 保留所有权利。
Borate-based bioactive glass scaffolds with a microstructure similar to that of human trabecular bone were prepared using a polymer foam replication method, and evaluated in vitro for potential bone repair applications. The scaffolds (porosity = 72 +/- 3%; pore size = 250-500 mu m) had a compressive strength of 6.4 +/- 1.0 MPa. The bioactivity of the scaffolds was confirmed by the formation of a hydroxyapatite (HA) layer on the surface of the glass within 7 days in 0.02 M K2HPO4 solution at 37 degrees C. The biocompatibility of the scaffolds was assessed from the response of cells to extracts of the dissolution products of the scaffolds, using assays of MTT hydrolysis, cell viability, and alkaline phosphatase activity. For boron concentrations below a threshold value (0.65 mM), extracts of the glass dissolution products supported the proliferation of bone marrow stromal cells, as well as the proliferation and function of murine MLO-A5 cells, an osteogenic cell line. Scanning electron microscopy showed attachment and continuous increase in the density of MLO-A5 cells cultured on the surface of the glass scaffolds. The results indicate that borate-based bioactive glass could be a potential scaffold material for bone tissue engineering provided that the boron released from the glass could be controlled below a threshold value. (C) 2009 Elsevier B.V. All rights reserved.