Application of Electron Paramagnetic Resonance (EPR) Oximetry to Monitor Oxygen in Wounds in Diabetic Models
Application of Electron Paramagnetic Resonance (EPR) Oximetry to Monitor Oxygen in Wounds in Diabetic Models
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DOI：
10.1371/journal.pone.0144914
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发表时间：
2015-12-14
期刊：
影响因子：
3.7
通讯作者：
Gallez, Bernard
中科院分区：
文献类型：
--
作者：
Desmet, Celine M.;Lafosse, Aurore;Gallez, Bernard
缺氧被经典地描述为糖尿病患者伤口愈合受损的主要原因。即使氧在伤口愈合过程中的作用得到了很好的认识，伤口中氧水平的测量仍然具有挑战性。本研究的目的是评价电子顺磁共振(EPR)血氧仪在糖尿病小鼠模型愈合过程中监测创面Po(2)的价值。用链脲佐菌素(STZ)处理小鼠和db/db小鼠，观察创面愈合的动力学。在两种不同的创面模型：全层切除皮肤创面和带蒂皮瓣中，以锂酞菁晶体为氧传感器，在愈合过程中重复进行Po(2)的1 GHz EPR波谱分析。与对照组(db/+)相比，12周龄db/db组小鼠伤口闭合动力学显著减慢，而STZ治疗组的伤口闭合动力学与对照组小鼠相比无统计学差异。在切除伤口的中心，在愈合过程的早期，测量受到大气氧气的高度影响。带蒂皮瓣在伤后早期出现缺氧现象。在db/+小鼠中，随着时间的推移发生复氧，而在糖尿病db/db模型中，缺氧时间延长。这一观察结果与活体显微镜观察到的愈合受损和微血管病变是一致的。总之，使用LiPc晶体作为氧传感器的EPR血氧测定仪是一种适合于跟踪急慢性创面、正常动物和糖尿病动物创面氧合的技术。然而，这项技术仅限于带蒂皮瓣的测量，不能应用于大气氧气扩散显著影响测量的切除创面。
A lack of oxygen is classically described as a major cause of impaired wound healing in diabetic patients. Even if the role of oxygen in the wound healing process is well recognized, measurement of oxygen levels in a wound remains challenging. The purpose of the present study was to assess the value of electron paramagnetic resonance (EPR) oximetry to monitor pO(2) in wounds during the healing process in diabetic mouse models. Kinetics of wound closure were carried out in streptozotocin (STZ)-treated and db/db mice. The pO(2) was followed repeatedly during the healing process by 1 GHz EPR spectroscopy with lithium phthalocyanine (LiPc) crystals used as oxygen sensor in two different wound models: a full-thickness excisional skin wound and a pedicled skin flap. Wound closure kinetics were dramatically slower in 12-week-old db/db compared to control (db/+) mice, whereas kinetics were not statistically different in STZ-treated compared to control mice. At the center of excisional wounds, measurements were highly influenced by atmospheric oxygen early in the healing process. In pedicled flaps, hypoxia was observed early after wounding. While reoxygenation occurred over time in db/+ mice, hypoxia was prolonged in the diabetic db/db model. This observation was consistent with impaired healing and microangiopathies observed using intravital microscopy. In conclusion, EPR oximetry using LiPc crystals as the oxygen sensor is an appropriate technique to follow wound oxygenation in acute and chronic wounds, in normal and diabetic animals. Nevertheless, the technique is limited for measurements in pedicled skin flaps and cannot be applied to excisional wounds in which diffusion of atmospheric oxygen significantly affects the measurements.