Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method
Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method
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DOI：
10.1073/pnas.0809851105
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发表时间：
2008-11-18
影响因子：
11.1
通讯作者：
Klibanov, Alexander M.
中科院分区：
文献类型：
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作者：
Antipov, Eugene;Cho, Art E.;Klibanov, Alexander M.
建立了一种基于酵母表面展示和荧光激活细胞分选（FACS）的高效酶对映体选择性筛选方法。其在辣根过氧化物酶中的应用已经产生了对任意底物对映体选择性高达2个数量级的酶变体。这些显着的改善，对映体选择性的表面结合和可溶性的酶证明和合理化的计算对接研究。
A highly efficient selection method for enhanced enzyme enantioselectivity based on yeast surface display and fluorescence-activated cell sorting (FACS) is developed and validated. its application to horseradish peroxidase has resulted in enzyme variants up to 2 orders of magnitude selective toward either substrate enantiomer at will. These marked improvements in enantioselectivity are demonstrated for the surface-bound and soluble enzymes and rationalized by computational docking studies.