Research on the interaction mechanism between quantum dots and radionuclides for the improvement of Cerenkov luminescence imaging
Research on the interaction mechanism between quantum dots and radionuclides for the improvement of Cerenkov luminescence imaging
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量子点与放射性核素相互作用机制研究改善切伦科夫发光成像
DOI：
10.1007/s11431-015-5897-x
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发表时间：
2015-10-01
影响因子：
4.6
通讯作者：
Zhai Peng
中科院分区：
文献类型：
--
作者：
Tang XiaoBin;Hou XiaoXiao;Zhai Peng
切伦科夫发光成像（CLI）是一种较新的光学分子成像技术。量子点的斯托克斯位移特性可以用来改善激光干涉仪的质量。然而，量子点与切伦科夫光的相互作用机制仍不清楚。本文研究了量子点与发射β射线、γ射线和切伦科夫光的放射性核素的相互作用机理。使用96孔板测试不同QD浓度的放射性核素的不同放射性水平。使用透明小瓶来确定QD荧光强度与从QD到放射性核素的距离之间的关系。此外，使用黑纸来阻挡切伦科夫光通过QD小瓶的透射。当QD浓度保持不变时，发现光子数与放射性核素的放射性之间存在线性关系。同样，当放射性核素的放射性保持恒定时，光子数与QD浓度呈线性相关。此外，随着放射性核素与量子点之间距离的增加，光子数呈指数下降。同时，量子点在切伦科夫光激发下发射的光子数占131I放射性核素激发光子总数的20%。结果表明，量子点不仅能被切伦科夫光激发，而且还能被其他光激发。
Cerenkov luminescence imaging (CLI) is a relatively new optical molecular imaging technique. The nature of Stokes shift in quantum dots (QD) can be used to improve the quality of CLI. However, the interaction mechanism of QD with Cerenkov light remains unclear. In this work, the interaction mechanism between QD and radionuclides emitting β rays, γ rays, and Cerenkov light was investigated. The 96-well plates were used to test the different levels of radioactivity of radionuclides with different QD concentrations. Transparent vials were used to determine the relationship between QD fluorescence intensity and the distance from QD to the radionuclide. In addition, black paper was used to block the transmission of Cerenkov light through the QD vials. A linear relationship was found between the number of photons and the radioactivity of radionuclides when the QD concentration was kept constant. Similarly, the number of photons was linearly related to the QD concentration when the radioactivity of radionuclides was kept constant. Furthermore, with the increases in the distance between radionuclides and quantum dots, the number of photons was exponentially decreased. Meanwhile, the number of photons emitted from QD excited by Cerenkov light accounted for 20% the total number of photons excited by131I radionuclide. The result proved that QD was not only excited by Cerenkov light but also by other rays.