Characterization and differential nuclear localization of Nopp140 and a novel Nopp140-like protein in trypanosomes
Characterization and differential nuclear localization of Nopp140 and a novel Nopp140-like protein in trypanosomes
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DOI：
10.1128/ec.5.5.876-879.2006
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发表时间：
2006-05-01
期刊：
影响因子：
--
通讯作者：
Gull, K.
中科院分区：
文献类型：
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作者：
Kelly, S.;Singleton, W.;Gull, K.
锥虫具有两种Nopp 140的同源物：典型的Nopp 140和Nopp 140样蛋白（TbNoLP），其中GAR结构域取代了C-末端SRP 40结构域。两者都被磷酸化并与RNA聚合酶I共免疫沉淀。每一个paradigm有一个独特的亚核定位，和TbNoLP的耗尽产生一个扩大的核仁中，TbNopp 140含有地区分散。NoLP蛋白的限制性发生模式反映了进化中一个有趣的趋同，暗示了核质小核仁核糖核蛋白穿梭的功能。
Trypanosomatids possess two homologues of Nopp140: a canonical Nopp140 and a Nopp140-like protein (TbNoLP) in which a GAR domain replaces the C-terminal SRP40 domain. Both are phosphorylated and coimmunoprecipitate with RNA polymerase I. Each paralogue has a distinct subnuclear localization, and depletion of TbNoLP produces an enlarged nucleolus in which TbNopp140-containing regions disperse. The restricted occurrence pattern of NoLP proteins reflects an intriguing convergence in evolution, suggestive of a function in nucleoplasmic small nucleolar ribonucleoprotein shuttling.