NEXT-GENERATION SEQUENCING APPLICATIONS FOR WHEAT CROP IMPROVEMENT
NEXT-GENERATION SEQUENCING APPLICATIONS FOR WHEAT CROP IMPROVEMENT
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DOI：
10.3732/ajb.1100309
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发表时间：
2012-02-01
影响因子：
3
通讯作者：
Edwards, David
中科院分区：
文献类型：
--
作者：
Berkman, Paul J.;Lai, Kaitao;Edwards, David
面包小麦(Triticum aestivum；Poaccae)是一种重要的农作物。以卡路里摄入量衡量，它提供了世界每日食物供应量的近20%，与大米提供的食物供应量相似。在过去的40年里，由于先进的农艺实践和通过选择育种改良的种质相结合，小麦的产量翻了一番。最近，产量增长没有那么戏剧性，如果要满足不断增长的人口的需求，小麦产量将需要显著提高。下一代测序(NGS)技术正在给生物学带来革命性的变化，并可用于解决植物生物学中的关键问题。这些技术可以产生基因组草稿序列，大大减少了传统技术的成本和时间框架。此外，NGS技术可用于评估基因结构和表达，重要的是，确定重要农艺性状背后的可遗传基因组变异。本文综述了小麦基因组和NGS技术，详细说明了NGS技术在小麦应用中的一些问题，并描述了NGS技术如何开始影响小麦作物改良。
Bread wheat (Triticum aestivum; Poaccae) is a crop plant of great importance. It provides nearly 20% of the world's daily food supply measured by calorie intake, similar to that provided by rice. The yield of wheat has doubled over the last 40 years due to a combination of advanced agronomic practice and improved germplasm through selective breeding. More recently, yield growth has been less dramatic, and a significant improvement in wheat production will be required if demand from the growing human population is to be met.Next-generation sequencing (NGS) technologies are revolutionizing biology and can be applied to address critical issues in plant biology. Technologies can produce draft sequences of genomes with a significant reduction to the cost and timeframe of traditional technologies. In addition, NGS technologies can be used to assess gene structure and expression, and importantly, to identify heritable genome variation underlying important agronomic traits.This review provides an overview of the wheat genome and NGS technologies, details some of the problems in applying NGS technology to wheat, and describes how NGS technologies are starting to impact wheat crop improvement.