Androgen receptors in human preadipocytes and adipocytes:: regional specificities and regulation by sex steroids
Androgen receptors in human preadipocytes and adipocytes:: regional specificities and regulation by sex steroids
复制标题
DOI：
10.1152/ajpcell.1998.274.6.c1645
复制
发表时间：
1998-06-01
影响因子：
5.5
通讯作者：
Giudicelli, Y
中科院分区：
文献类型：
--
作者：
Dieudonné, MN;Pecquery, R;Giudicelli, Y
各种临床和流行病学证据有力地表明，性类固醇激素在确定人体脂肪分布的解剖学特异性方面发挥着重要作用。到目前为止，还没有研究检查雄激素受体（AR）在人类脂肪细胞和前脂肪细胞中的可能存在。我们研究了中年男性和女性在增殖和分化过程（脂肪形成）中来自不同解剖位置（腹内和皮下）的前脂肪细胞中的AR。在全细胞提取物中，通过[H-3]R-1881特异性结合定量的雄激素结合位点在腹腔内是皮下前脂肪细胞的两倍，但在男性和女性的相同脂肪库中相同。蛋白质印迹分析显示：1）AR存在于人前脂肪细胞的核和胞质组分中，2）脂肪形成过程中AR表达减少，3）体外雄激素对AR的上调。RT-PCR实验表明，AR mRNA在人前脂肪细胞和脂肪细胞中的存在，以及AR分布的区域特异性。然而，AR mRNA的表达被发现在脂肪形成过程中增加。在大鼠前脂肪细胞中观察到相同的结果。总之，这项研究清楚地表明，AR在人类前脂肪细胞和脂肪细胞的存在，并表明雄激素可能有助于，通过调节自己的受体，控制脂肪组织的发展。
Various clinical and epidemiological evidence strongly suggests a major role for sex steroid hormones in the determination of anatomical specificities of fat distribution in human. To date, no studies have examined the possible presence of androgen receptors (AR) in human adipocytes and preadipocytes. We, have studied AR in preadipocytes from various anatomical locations (intra-abdominal and subcutaneous) in middle-aged men and women during the proliferation and differentiation processes (adipogenesis). Androgen binding sites quantified by [H-3]R-1881-specific binding in whole cell extracts were twofold higher in intraabdominal than in subcutaneous preadipocytes but identical for the same fat depots in men and women. Western blot analysis revealed 1) the presence of AR in the nuclear and cytosolic fractions of human preadipocytes, 2) a decrease of AR expression during adipogenesis, and 3) an upregulation of AR by androgens in vitro. RT-PCR experiments showed the presence of AR mRNA in human preadipocytes and adipocytes and also the regional specificity of AR distribution. However, AR mRNA expression was found to increase during adipogenesis. The same results were observed in rat preadipocytes. In conclusion, this study clearly demonstrates the presence of AR in human preadipocytes and adipocytes and suggests that androgens may contribute, through regulation of their own receptors, to the control of adipose tissue development.