AUTORADIOGRAPHIC LOCALIZATION OF INSULIN-RECEPTORS IN RAT-BRAIN - PROMINENCE IN OLFACTORY AND LIMBIC AREAS
AUTORADIOGRAPHIC LOCALIZATION OF INSULIN-RECEPTORS IN RAT-BRAIN - PROMINENCE IN OLFACTORY AND LIMBIC AREAS
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DOI：
10.1016/0306-4522(86)90082-5
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发表时间：
1986-04-01
期刊：
影响因子：
3.3
通讯作者：
ROTH, J
中科院分区：
文献类型：
--
作者：
HILL, JM;LESNIAK, MA;ROTH, J
用放射自显影法显示了125 I标记的胰岛素在冷冻新鲜大鼠脑薄切片中的结合（95%特异性结合）。通过包括结构-活性关系分析在内的几个标准，脑胰岛素受体与先前在脑和其他更典型的靶组织上表征的胰岛素受体定性上无法区分，并且与胰岛素样生长因子的受体不同。脑内125 I标记的胰岛素结合位点形成了一种独特的模式，在所有的嗅觉区和密切相关的边缘区都有高水平的结合。在基底神经节和小脑的新皮层和辅助运动区也有显著的结合。在辅助脑结构中，只有脉络丛显示出高密度的胰岛素受体。当胰岛素受体位于皮质或层压结构上时，最高结合发生在浅表（“分子”或“丛状”）层，即接收传入输入和/或富含主要神经元树突分支的层（例如嗅球的外部丛状层和齿状回的分子层）。胰岛素受体在嗅觉系统和边缘系统中的富集，这是其他已知神经肽的特征，除了它们在主神经元的树突区域所占据的层中的普遍性之外，还表明胰岛素在脑中的神经调节功能。
The binding of 125I-labeled insulin in thin sections of frozen fresh rat brain (95% specific binding) was shown using autoradiography. By several criteria including structure-activity relationship analysis, the brain insulin receptors were qualitatively indistinguishable from insulin receptors previously characterized on brain and other more typical target tissues and distinct from receptors for the insulin-like growth factors. The 125I-labeled insulin binding sites in brain formed a distinct pattern with high levels of binding in all olfactory areas and in closely related limbic region. Binding was also prominent in the neocortex and the accessory motor areas of the basal ganglia and the cerebellum. Among ancillary brain structures only the choroid plexus demonstrated a high density of insulin receptors. When insulin receptors were located on cortical or laminated structures, the highest binding occurred in the superficial ("molecular" or "plexiform") layer, i.e. the layer which receives afferent input and/or is rich in the dendritic branches of principle neurons (e.g. external plexiform layer of the olfactory bulb and the molecular layer of the dentate gyrus). The enrichment of insulin receptors in the olfactory and limbic systems, which is a characteristic feature of other known neuropeptides, in addition to their prevalence in the strata occupied by the dendritic fields of principle neurons, suggests a neuromodulatory function for insulin in the brain.