Development of an efficient glucosinolate extraction method.
Development of an efficient glucosinolate extraction method.
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DOI：
10.1186/s13007-017-0164-8
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发表时间：
2017
期刊：
影响因子：
5.1
通讯作者：
Hartley SE
中科院分区：
文献类型：
--
作者：
Doheny-Adams T;Redeker K;Kittipol V;Bancroft I;Hartley SE
硫代葡萄糖苷是一种富含硫的阴离子次生代谢物，因其在农业上具有重要意义的十字花科植物中的存在及其对人类和动物健康的影响而被广泛研究。有毒硫代葡萄糖苷水解物的生物熏蒸性能作为一种控制农业害虫的方法也越来越引起人们的兴趣。评估生物熏蒸潜力需要快速准确地定量硫代葡萄糖苷，但目前常用的分析前提取方法涉及一些耗时和危险的步骤；本研究旨在开发一种改进的硫代葡萄糖苷提取方法。对以往从十字花科植物组织中提取硫代葡萄糖苷的三种方法，即冷甲醇提取法、煮沸甲醇提取法和沸水提取法进行了比较，并对其组织类型(根、茎叶)和四种十字花科植物(芥菜、白芥菜、萝卜和十字花科植物)进行了比较。除葡萄糖苷外，冷甲醇提取法提取硫代葡萄糖苷的效果与其他方法相同或更好。研究还表明，在提取过程中经常使用的冻干方法可以降低硫代葡萄糖苷的最终浓度，从冷冻的湿组织样本中提取80%的甲醇更有效。我们提出了一种从植物组织中提取硫代葡萄糖苷的简化方法，该方法不需要使用冷冻干燥器或沸腾的甲醇，因此危害更小，时间更长，成本效益更高。已经证明，与所研究的十字花科植物中主要硫代葡萄糖苷的常用ISO方法相比，本方法具有类似或改进的硫代葡萄糖苷提取效率：芥菜中的芥子苷和葡萄糖苷；白芥菜中的芥子苷、葡萄糖苷和葡萄糖苷；萝卜中的葡萄糖酚和葡萄糖苷；以及十字花科植物中的硫代葡萄糖苷、葡萄糖芥素和葡萄糖萝卜素。本文的在线版本(doi：10.1186/s13007-0170164-8)包含补充材料，授权用户可以使用。
Glucosinolates, anionic sulfur rich secondary metabolites, have been extensively studied because of their occurrence in the agriculturally important brassicaceae and their impact on human and animal health. There is also increasing interest in the biofumigant properties of toxic glucosinolate hydrolysis products as a method to control agricultural pests. Evaluating biofumigation potential requires rapid and accurate quantification of glucosinolates, but current commonly used methods of extraction prior to analysis involve a number of time consuming and hazardous steps; this study aimed to develop an improved method for glucosinolate extraction. Three methods previously used to extract glucosinolates from brassicaceae tissues, namely extraction in cold methanol, extraction in boiling methanol, and extraction in boiling water were compared across tissue type (root, stem leaf) and four brassicaceae species (B. juncea, S. alba, R. sativus, and E. sativa). Cold methanol extraction was shown to perform as well or better than all other tested methods for extraction of glucosinolates with the exception of glucoraphasatin in R. sativus shoots. It was also demonstrated that lyophilisation methods, routinely used during extraction to allow tissue disruption, can reduce final glucosinolate concentrations and that extracting from frozen wet tissue samples in cold 80% methanol is more effective. We present a simplified method for extracting glucosinolates from plant tissues which does not require the use of a freeze drier or boiling methanol, and is therefore less hazardous, and more time and cost effective. The presented method has been shown to have comparable or improved glucosinolate extraction efficiency relative to the commonly used ISO method for major glucosinolates in the Brassicaceae species studied: sinigrin and gluconasturtiin in B. juncea; sinalbin, glucotropaeolin, and gluconasturtiin in S. alba; glucoraphenin and glucoraphasatin in R. sativus; and glucosatavin, glucoerucin and glucoraphanin in E. sativa. The online version of this article (doi:10.1186/s13007-017-0164-8) contains supplementary material, which is available to authorized users.