Protein identification using MS/MS data
Protein identification using MS/MS data
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DOI：
10.1016/j.jprot.2011.05.014
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发表时间：
2011-09-06
影响因子：
3.3
通讯作者：
Cottrell, John S.
中科院分区：
文献类型：
--
作者：
Cottrell, John S.
本教程的主题是通过MS/MS数据的数据库搜索进行蛋白质鉴定和表征。多肽质量指纹图谱被排除在外，因为它在单独的教程中介绍。重点介绍了数据库检索的实用方面，如序列数据库的选择、质量公差的影响以及如何识别翻译后修饰。讨论了灵敏度和特异度之间的关系，以及使用肽匹配信息来推断样本中存在哪些蛋白质的挑战。由于这些教程本质上是介绍性的，大多数参考文献是综述，而不是主要的研究论文。假定对质谱学和蛋白质化学有一定的了解。还有一张幻灯片演示文稿，包括演讲者笔记，以及一系列旨在强调要点的基于网络的实践练习。本教程是国际蛋白质组学教程计划(IPTp 6)的一部分。(C)2011爱思唯尔B.V.保留所有权利。
The subject of this tutorial is protein identification and characterisation by database searching of MS/MS Data. Peptide Mass Fingerprinting is excluded because it is covered in a separate tutorial. Practical aspects of database searching are emphasised, such as choice of sequence database, effect of mass tolerance, and how to identify post-translational modifications. The relationship between sensitivity and specificity is discussed, as is the challenge of using peptide match information to infer which proteins were present in the sample.Since these tutorials are introductory in nature, most references are to reviews, rather than primary research papers. Some familiarity with mass spectrometry and protein chemistry is assumed. There is an accompanying slide presentation, including speaker notes, and a collection of web-based, practical exercises, designed to reinforce key points. This Tutorial is part of the International Proteomics Tutorial Programme (IPTP 6). (C) 2011 Elsevier B.V. All rights reserved.