Electrically tunable lenses - eliminating mechanical axial movements during high-speed 3D live imaging.
Electrically tunable lenses - eliminating mechanical axial movements during high-speed 3D live imaging.
复制标题
DOI：
10.1242/jcs.258650
复制
发表时间：
2021-08
影响因子：
4
通讯作者：
Christoforos Efstathiou;Viji M. Draviam
中科院分区：
文献类型：
--
作者：
Christoforos Efstathiou;Viji M. Draviam
对光敏和动态生物事件的成功研究，例如在增殖组织或分裂细胞中的那些事件，需要非介入的高速成像技术。电可调透镜(ETL)是一种具有形状变化能力的液体透镜，能够实现焦平面的快速轴向移动，进而在毫秒范围内实现采集速度。这些受人眼启发的液体透镜可以实现快速对焦，并已被应用于各种细胞生物学研究。在这里，我们回顾了使用高性价比高速ETL的历史、机遇和挑战。尽管有其他更昂贵的毫秒三维成像解决方案可用，但ETL仍然是活细胞显微镜的强大而廉价的竞争者。
The successful investigation of photosensitive and dynamic biological events, such as those in a proliferating tissue or a dividing cell, requires non-intervening high-speed imaging techniques. Electrically tunable lenses (ETLs) are liquid lenses possessing shape-changing capabilities that enable rapid axial shifts of the focal plane, in turn achieving acquisition speeds within the millisecond regime. These human-eye-inspired liquid lenses can enable fast focusing and have been applied in a variety of cell biology studies. Here, we review the history, opportunities and challenges underpinning the use of cost-effective high-speed ETLs. Although other, more expensive solutions for three-dimensional imaging in the millisecond regime are available, ETLs continue to be a powerful, yet inexpensive, contender for live-cell microscopy.