Alternating block polyurethanes based on PCL and PEG as potential nerve regeneration materials
Alternating block polyurethanes based on PCL and PEG as potential nerve regeneration materials
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基于PCL和PEG的交替嵌段聚氨酯作为潜在的神经再生材料
DOI：
10.1002/jbm.a.34732
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发表时间：
2014-03-01
影响因子：
4.9
通讯作者：
Xu, Kaitian
中科院分区：
文献类型：
--
作者：
Li, Guangyao;Li, Dandan;Xu, Kaitian
通过聚己内酯二醇与末端二异氰酸酯聚乙二醇的选择性偶联反应，制备了具有规则和可控制嵌段排列的聚己内酯（PCL-diol）和聚乙二醇（PEG）的交联嵌段聚氨酯（简称pcl - et -PEG）。通过FTIR、H-1 NMR、GPC、DSC和TGA等手段对其化学结构、分子量、分布和热性能进行了系统表征。通过H2O和CH2I2的静态接触角研究了其亲水性。采用扫描电镜（SEM）和原子力显微镜（atomic force microscope）对薄膜表面进行了观察，并用万能试验机对薄膜的力学性能进行了测试。结果表明，与随机嵌段聚氨酯相比，交替嵌段聚氨酯具有更高的结晶度、更高的力学性能、更亲水性和更粗糙的表面（深沟）。血小板粘附表明pcl -alt-PEG具有较好的血液相容性，且其血液相容性受PEG含量的显著影响。PEG含量高，具有良好的血液相容性。CCK-8实验和扫描电镜观察表明，成纤维细胞L929与大鼠胶质细胞在交替阻滞聚氨酯上的相容性明显优于随机阻滞聚氨酯。交替块聚氨酯PUC20-a-E4具有优化的成分、机械、表面性能、血液相容性和最高的细胞生长和增殖能力，在神经再生中具有潜在的应用前景。(c) 2013 Wiley Periodicals, Inc.；[J] .中国生物医学工程学报，2016,31(2):559 - 567。
Polyurethanes with regular and controlled block arrangement, i.e., alternating block polyurethanes (abbreviated as PUCL-alt-PEG) based on poly(epsilon-caprolactone) (PCL-diol) and poly(ethylene glycol) (PEG) was prepared via selectively coupling reaction between PCL-diol and diisocyanate end-capped PEG. Chemical structure, molecular weight, distribution, and thermal properties were systematically characterized by FTIR, H-1 NMR, GPC, DSC, and TGA. Hydrophilicity was studied by static contact angle of H2O and CH2I2. Film surface was observed by scanning electron microscope (SEM) and atomic force microscopy, and mechanical properties were assessed by universal test machine. Results show that alternating block polyurethanes give higher crystal degree, higher mechanical properties, and more hydrophilic and rougher (deep ravine) surface than their random counterpart, due to regular and controlled structure. Platelet adhesion illustrated that PUCL-alt-PEG has better hemocompatibility and the hemacompatibility was affected significantly by PEG content. Excellent hemocompatibility was obtained with high PEG content. CCK-8 assay and SEM observation revealed much better cell compatibility of fibroblast L929 and rat glial cells on the alternating block polyurethanes than that on random counterpart. Alternating block polyurethane PUC20-a-E4 with optimized composition, mechanical, surface properties, hemacompatibility, and highest cell growth and proliferation was achieved for potential use in nerve regeneration. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 685-697, 2014.