Age and isolation influence steroids release and chemical signaling in male mice
Age and isolation influence steroids release and chemical signaling in male mice
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DOI：
10.1016/j.steroids.2014.01.009
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发表时间：
2014-05-01
期刊：
影响因子：
2.7
通讯作者：
Gabai, Gianfranco
中科院分区：
文献类型：
--
作者：
Mucignat-Caretta, Carla;Cavaggioni, Andrea;Gabai, Gianfranco
老鼠的社会互动涉及嗅觉信号，它传达了关于发射器的信息。反过来，小鼠的社会和生理状态可能会改变化学线索的释放。在这项研究中，年龄和社会隔离对内分泌反应和化学信号释放的影响在雄性CD 1小鼠中进行了研究，这些小鼠被分为四组：（从断奶至60天; N= 6），成年隔离（直到180天; N= 6），幼年组（6只小鼠/笼;直到60天; N= 18），成年组（6只小鼠/笼;直到180天; N= 18）。将小鼠转移到干净的笼子中以观察排尿模式，然后处死。测量体重和器官重量、血清睾酮、脱氢表雄酮、皮质酮和主要尿蛋白/肌酐比值。尿挥发性分子可能参与信息素通信进行了鉴定。雄激素分泌在隔离的小鼠中更高（P< 0.05），表明下丘脑-垂体-性腺轴的反应性更高。分组小鼠表现出更高程度的肾上腺活性，年轻小鼠表现出更高的血清皮质酮（P < 0.05），表明对下丘脑-肾上腺-肾上腺轴的刺激更大。仅在幼龄分离小鼠中观察到显性雄性的典型排尿模式，包括排出大量液滴，其显示较高的蛋白/肌酐比（P< 0.05）。青年和成年隔离鼠尿中2-s-丁基噻唑啉含量均高于成年隔离鼠（P< 0.005）。年轻的隔离小鼠在排尿模式和潜在活性物质排放方面表现出最显著的差异，而长期隔离导致不太极端的表型;因此，社会隔离对年轻小鼠激素和信息素释放有更大的影响。(C)2014爱思唯尔公司All rights reserved.
Social interactions in mice involve olfactory signals, which convey information about the emitter. In turn, the mouse social and physiological status may modify the release of chemical cues. In this study, the influences of age and social isolation on the endocrine response and the release of chemical signals were investigated in male CD1 mice, allocated into four groups: Young Isolated (from weaning till 60 days; N= 6), Adult Isolated (till 180 days; N= 6), Young Grouped (6 mice/cage; till 60 days; N= 18), Adult Grouped (6 mice/cage; till 180 days; N= 18). Mice were transferred in a clean cage to observe the micturition pattern and then sacrificed. Body and organs weights, serum testosterone, dehydroepiandrosterone, corticosterone and the ratio Major Urinary Protein/creatinine were measured. Urinary volatile molecules potentially involved in pheromonal communication were identified. Androgen secretion was greater in isolated mice (P< 0.05), suggesting a greater reactivity of the Hypothalamic-Pituitary-Gonadal axis. Grouped mice presented a higher degree of adrenal activity, and young mice showed a higher serum corticosterone (P < 0.05) suggesting a greater stimulation of the Hypothalamic-Pituitary-Adrenal axis. The micturition pattern typical of dominant male, consisting in voiding numerous droplets, was observed in Young Isolated mice only, which showed a higher protein/creatinine ratio (P< 0.05). Urinary 2-s-butyl-thiazoline was higher in both Young and Adult Isolated mice (P< 0.005). Young Isolated mice showed the most prominent difference in both micturition pattern and potentially active substance emission, while long term isolation resulted in a less extreme phenotype; therefore social isolation had a higher impact on young mice hormone and pheromone release. (C) 2014 Elsevier Inc. All rights reserved.