Isolation, structure, and surfactant properties of polysaccharides fromUlva lactuca L. from South China Sea
Isolation, structure, and surfactant properties of polysaccharides fromUlva lactuca L. from South China Sea
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南海石莼多糖的分离、结构和表面活性剂性质
DOI：
10.1016/j.ijbiomac.2015.05.031
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发表时间：
2015
影响因子：
8.2
通讯作者：
Junhua Chen
中科院分区：
文献类型：
--
作者：
Hua Tian;Xueqiong Yin;Qinghuan Zeng;Li zhu;Junhua Chen
Two polysaccharides (ULP1 and ULP2) were isolated through ultrasonic-assisted extraction from green seaweedUlva lactucaL. which was collected from the South China Sea. The highest yield of 17.57% was obtained under the conditions of 2% NaOH, 90 °C, material/water mass ratio 1:80, liquid extraction 5 h and subsequent ultrasound-assisted extraction 1 h. The structure of ULPs were characterized with periodate oxidation followed by Smith degradation,1H NMR,13C NMR spectroscopy, FTIR, and GPC. The molecular weights of ULP1 and ULP2 were 189 kDa and 230 kDa, respectively. The structural characteristics of ULP1 and ULP2 were quite similar. They were composed of rhamnose, xylose, glucose, and glucuronic acid. The content of rhamnose, xylose, glucose, glucuronic acid, sulfate was 51.2%, 12.3%, 20.1%, 16.4%, 12.0% for ULP1, respectively, and 60.8%, 14.2%, 8.2%, 16.8%, 26.8%, respectively, for ULP2. Both ULP1 and ULP2 showed good surface activity. 5 mg/mL ULP1 (2.62 × 10−2mmol/L) decreased the water surface tension to 51.63 mN/m. The critical micellar concentration of ULP1 and ULP2 was 1.01 mg/mL (5.3 × 10−3mmol/L) and 1.14 mg/mL (5.0 × 10−3mmol/L), respectively.
