Diagnostic Significance of Serum IgG Galactosylation in CA19-9-Negative Pancreatic Carcinoma Patients
Diagnostic Significance of Serum IgG Galactosylation in CA19-9-Negative Pancreatic Carcinoma Patients
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CA19-9阴性胰腺癌患者血清IgG半乳糖基化的诊断意义
DOI：
10.3389/fonc.2019.00114
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发表时间：
2019-02
影响因子：
4.7
通讯作者：
Gu Jianxin
中科院分区：
文献类型：
--
作者：
Zhong Ailing;Qin Ruihuan;Qin Wenjun;Han Jing;Gu Yong;Zhou Lei;Zhang Hongqin;Ren Shifang;Lu Renquan;Guo Lin;Gu Jianxin
背景资料：尽管糖类抗原19-9（CA 19 -9）被认为是胰腺癌（PC）的临床有用和信息，但假阳性结果和假阴性结果限制了其临床应用。尤其是CA 19 -9阴性的PC患者的漏诊或延迟诊断限制了其实用性。为了改善PC患者的预后，需要发现可靠的生物标志物来辅助CA 19 -9。血清IgG半乳糖基化的基础上，我们以前的报告改变PC患者相比，健康对照。本研究旨在探讨IgG半乳糖基化在辅助CA 19 -9综合诊断PC中的意义。研究方法：通过MALDI-MS分析队列1（n = 252）和队列2（其中所有CA 19 -9水平均为阴性）中的血清IgG半乳糖基化谱（n = 133）。在每个队列中，不仅包括健康对照和PC患者，而且包括良性胰腺疾病（BPD）患者。通过MALDI-MS样品采集软件采集峰，然后通过Progenesis MALDI软件进行处理和分析。根据公式（G 0/（G1+G2×2）），根据峰G 0、G1和G2的相对强度计算IgG半乳糖基化比率，将其用作指示IgG半乳糖基化分布的指标。结果：与非癌组（健康对照组和BPD组）相比，PC组的Gal-比值升高。IgG Gal-比率的受试者工作特征曲线下面积（AUC）高于CA 19 -9的受试者工作特征曲线下面积（0.912 vs. 0.814）。当Gal-比率和CA 19 -9组合时，性能进一步改善（AUC：0.928）。同时，半乳糖比值对前列腺癌的早期诊断也有较高的诊断价值，其敏感性为92.31%（AUC：0.883）。值得注意的是，IgG Gal比率在CA 19 -9阴性PC患者中具有很高的灵敏度（90.63%）和特异性（76.81%）。结论：IgG Gal-比率在PC的检测中具有很好的性能，并且可以用于辅助CA 19 -9通过早期检测、与BPD的鉴别以及在CA 19 -9阴性水平下的PC诊断来提高诊断性能。
Background: Although Carbohydrate antigen 19-9 (CA19-9) is considered clinically useful and informative for pancreatic carcinoma (PC), false positive results, and false negative results have restricted its clinical use. Especially missed or delayed diagnosis of PC patients with negative CA19-9 value limited the utility. To improve prognosis of PC patients, the discovery of reliable biomarkers to assist CA19-9 is desired. Serum IgG galactosylation based on our previous report was altered in PC patients comparing to healthy controls. The objective of this study was to explore the diagnostic significance of IgG galactosylation in assisting CA19-9 for PC in a comprehensive way. Methods: Serum IgG galactosylation profiles were analyzed by MALDI-MS in cohort 1 (n = 252) and cohort 2 in which all CA19-9 levels were negative (n = 133). In each cohort, not only healthy controls and PC patients but also benign pancreatic disease (BPD) patients were enrolled. Peaks were acquired by the software of MALDI-MS sample acquisition, followed by being processed and analyzed by the software of Progenesis MALDI. IgG Gal-ratio, which was calculated from the relative intensity of peaks G0, G1, and G2 according to the formula (G0/(G1+G2×2)), was employed as an index for indicating the distribution of IgG galactosylation. Results: The Gal-ratio was elevated in PC comparing with that in non-cancer group (healthy controls and BPD). The area under the receiver operating characteristic curve (AUC) of IgG Gal-ratio was higher than that of CA19-9 (0.912 vs. 0.814). The performance was further improved when Gal-ratio and CA19-9 were combined (AUC: 0.928). Meanwhile, Gal-ratio also had great diagnostic value with a sensitivity of 92.31% (AUC: 0.883) in detection of PC at early stage. Notably, IgG Gal-ratio has great sensitivity (90.63%) and specificity (76.81%) in CA19-9-negative PC patients. Conclusions: IgG Gal-ratio had a great performance in detection of PC and could be used to assist CA19-9 in improving diagnosis performance through early stage detection, differentiation from BPD, and PC diagnosis with CA19-9-negative level.
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DOI：
10.1891/9780826121646.0002
发表时间：
2018-09
期刊：
Cancer Rehabilitation
影响因子：
--
作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
通讯作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
DOI：
10.1093/jnci/djt439
发表时间：
2014-02
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Jabbar KS;Verbeke C;Hyltander AG;Sjövall H;Hansson GC;Sadik R
通讯作者：
Sadik R
基于质谱的 N-糖蛋白组学用于癌症生物标志物发现。
影响因子：
3.8
作者：
Zhang Y;Jiao J;Yang P;Lu H
通讯作者：
Lu H
DOI：
10.1074/mcp.m111.010090
发表时间：
2011-10
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Pucić M;Knezević A;Vidic J;Adamczyk B;Novokmet M;Polasek O;Gornik O;Supraha-Goreta S;Wormald MR;Redzić I;Campbell H;Wright A;Hastie ND;Wilson JF;Rudan I;Wuhrer M;Rudd PM;Josić D;Lauc G
通讯作者：
Lauc G
影响因子：
2.9
作者：
Cabrales P;Salazar Vázquez MA;Salazar Vázquez B;Rodríguez-Morán M;Intaglietta M;Guerrero-Romeros F
通讯作者：
Guerrero-Romeros F