Inactivation of photosensitizing merocyanine dyes by plasma, serum and serum components
Inactivation of photosensitizing merocyanine dyes by plasma, serum and serum components
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DOI：
10.1111/j.1751-1097.1996.tb03124.x
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发表时间：
1996-10-01
影响因子：
3.3
通讯作者：
Sieber, F
中科院分区：
文献类型：
--
作者：
Anderson, GS;Gunther, WHH;Sieber, F
在电子给体杂环中具有氧的汞染料被血浆、血清和血清组分快速降解，氧被硫或硒原子取代使染料难以降解，不稳定汞染料的降解是温度依赖性的而不依赖于氧，负责汞染料降解的血浆组分对热和去污剂敏感，说明是酶促反应鉴定对降解的敏感性/抗性的结构要求为实验者提供了一种简单的方法来操纵部花青在高血清或血浆环境中的稳定性，并且可以将汞光敏剂的临床用途扩展到其在骨髓移植物的体外净化中的传统作用之外。
Merocyanine dyes with an oxygen in the electron donor heterocycle were rapidly degraded by plasma, serum and serum components, Replacement of the oxygen by a sulfur or selenium atom rendered the dyes refractory to degradation, The degradation of labile merocyanine dyes was temperature dependent and oxygen independent, The plasma component that was responsible for the degradation of merocyanine dyes was sensitive to heat and detergent, suggesting an enzymatic process. The identification of the structural requirements for sensitivity/resistance to degradation provides the experimenter with a simple means to manipulate the stability of merocyanines in high serum or plasma environments and may expand the clinical utility of merocyanine photosensitizers beyond their traditional role in the extracorporeal purging of bone marrow grafts.