BRAIN ADENOSINE CONCENTRATION DURING HYPOXIA IN RATS
BRAIN ADENOSINE CONCENTRATION DURING HYPOXIA IN RATS
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DOI：
10.1152/ajpheart.1981.241.2.h235
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发表时间：
1981-01-01
影响因子：
--
通讯作者：
BERNE, RM
中科院分区：
文献类型：
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作者：
WINN, HR;RUBIO, R;BERNE, RM
在大鼠急性缺氧（30 s）和持续缺氧（5 min）和低碳酸血症期间，分析脑组织中腺苷及其代谢产物肌苷和次黄嘌呤。在缺氧开始的30秒内，腺苷水平从0.34 ± 0.001增加到0.34 ± 0.001。0.08（SE）至1.65 .+-。0.33 nmol/g，与脑血流量的变化在时间上相平行。在持续缺氧和低碳酸期间，通过快速（冷冻-吹）或缓慢（原位）冷冻方法对脑组织进行取样。使用冷冻吹扫技术，腺苷浓度在动脉血氧分压（PaO 2）> 200和100 mm Hg之间保持稳定，在PaO 2 = 50 mm Hg时增加一倍，当PaO 2达到30 mm Hg时增加7倍。原位技术未观察到腺苷或其代谢产物增加。在低碳酸血症（动脉CO2分压< 20 mm Hg）期间，两种采样技术的腺苷浓度均升高。在原位冷冻期间测量脑中的冷冻时间，并且在缺氧期间增加，在低碳酸期间减少。缺氧时脑内腺苷浓度增加。在大鼠中的原位技术似乎不适合于在脑血流量增加的条件下对脑组织进行采样以用于随后的腺苷测量。
Brain tissue was analyzed for adenosine and its metabolites, inosine and hypoxanthine, in rats during acute (30 s) hypoxia and during sustained (5 min) hypoxia and hypocarbia. Within 30 s of the onset of hypoxia, adenosine levels were increased from 0.34 .+-. 0.08 (SE) to 1.65 .+-. 0.33 nmol/g, paralleling temporally the changes in cerebral blood flow. During sustained hypoxia and hypocarbia, brain tissue was sampled by a fast (freeze-blow) or slow (in situ) freezing method. With the freeze-blow technique, adenosine concentrations remained stable between arterial partial pressure of O2 (PaO2) > 200 and 100 mm Hg, doubled at PaO2 = 50 mm Hg and increased 7-fold when PaO2 reached 30 mm Hg. No increases in adenosine or its metabolites were noted with the in situ technique. During hypocarbia (arterial CO2 partial pressure < 20 mm Hg), adenosine concentrations increased with both sampling techniques. Freezing times in brain were measured during in situ freezing and were increased during hypoxia and decreased during hypocarbia. Adenosine concentrations in brain are increased during hypoxia. The in situ technique in rat does not appear to be optimal for sampling brain tissue for subsequent measurement of adenosine under conditions where cerebral blood flow is increased.