Effects of caffeine on the uncoupling protein family in obese yellow KK mice
Effects of caffeine on the uncoupling protein family in obese yellow KK mice
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DOI：
10.1046/j.1440-1681.2002.03675.x
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发表时间：
2002-05-01
影响因子：
2.9
通讯作者：
Yoshida, T
中科院分区：
文献类型：
--
作者：
Kogure, A;Sakane, N;Yoshida, T
1. 我们在肥胖小鼠中研究了咖啡因上调解偶联蛋白(UCP)-1、UCP-2和UCP-3表达的假设，这些蛋白参与了产热。采用实时定量逆转录-聚合酶链式反应法测定肥胖黄色KK小鼠皮下给药60 mg/kg咖啡因或生理盐水4 h后，棕色脂肪组织（BAT）、白色脂肪组织（WAT）和骨骼肌中UCP-1、-2和-3 mRNA水平。同时测定血浆游离脂肪酸、肾上腺素、去甲肾上腺素和多巴胺水平。在注射咖啡因的肥胖小鼠中，与注射生理盐水的对照小鼠相比，BAT中UCP-1 mRNA水平显著增加了1.5倍，BAT和骨骼肌中UCP-2 mRNA水平分别增加了1.8倍和2.5倍，BAT和骨骼肌中UCP-3 mRNA水平分别增加了1.7倍和3.4倍。WAT中UCP-2 mRNA水平在两组间无明显差异。与注射生理盐水的小鼠相比，注射咖啡因的小鼠血浆游离脂肪酸和肾上腺素水平显著升高。综上所述，咖啡因上调BAT中UCP-1、UCP-2和UCP-3以及骨骼肌中UCP-2和UCP-3的表达，可能参与肥胖小鼠的产热作用。
1. The hypothesis that caffeine upregulates uncoupling protein (UCP)-1, UCP-2 and UCP-3 expression, which contribute to thermogenesis, was investigated in obese mice.2. The mRNA levels of UCP-1, -2 and -3 in brown adipose tissue (BAT), UCP-2 in white adipose tissue (WAT), and UCP-2 and -3 in skeletal muscle were measured using real-time quantitative reverse transcription-polymerase chain reaction analysis in obese yellow KK mice 4 h after the subcutaneous administration of either 60 mg/kg caffeine or physiological saline. Plasma free fatty acids, adrenaline, noradrenaline and dopamine levels were also measured.3. In caffeine-injected obese mice, UCP-1 mRNA levels were significantly increased by 1.5-fold in BAT, UCP-2 mRNA levels were increased by 1.8- and 2.5-fold in BAT and skeletal muscles, respectively, and UCP-3 mRNA levels were increased 1.7- and 3.4-fold in BAT and skeletal muscles, respectively, compared with control mice injected with physiological saline. There was no difference in UCP-2 mRNA levels in WAT between the two groups.4. Plasma free fatty acids and adrenaline levels were significantly elevated in mice treated with caffeine compared with those injected with physiological saline.5. It was concluded that caffeine upregulates the expression of UCP-1, UCP-2 and UCP-3 in BAT and UCP-2 and UCP-3 in skeletal muscles, which may contribute to thermogenesis in obese mice.