Dynamic light scattering optical coherence tomography
Dynamic light scattering optical coherence tomography
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DOI：
10.1364/oe.20.022262
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发表时间：
2012-09-24
期刊：
影响因子：
3.8
通讯作者：
Boas, David A.
中科院分区：
文献类型：
--
作者：
Lee, Jonghwan;Wu, Weicheng;Boas, David A.
我们引入了动态光散射 (DLS) 和光学相干断层扫描 (OCT) 的集成，用于异质扩散和流动的高分辨率 3D 成像。 DLS 分析颗粒散射光的波动，以测量颗粒的扩散或流动，OCT 使用相干门控收集仅从小体积散射的光，以进行高分辨率结构成像。因此，DLS 和 OCT 的集成可以实现扩散和流动的高分辨率 3D 成像。我们在假设静态和移动粒子在 OCT 分辨率体积内混合并且移动粒子可以表现出扩散或平移运动的假设下得出了一个理论。基于这一理论，我们开发了一种拟合算法来估计动态参数，包括轴向和横向速度以及扩散系数。我们通过数值模拟和模型实验验证了扩散和流动的 DLS-OCT 测量。作为示例应用，我们对活体动物大脑进行了 DLS-OCT 成像，生成了绝对速度和轴向速度、扩散系数和决定系数的 3D 图。 (C) 2012年美国光学学会
We introduce an integration of dynamic light scattering (DLS) and optical coherence tomography (OCT) for high-resolution 3D imaging of heterogeneous diffusion and flow. DLS analyzes fluctuations in light scattered by particles to measure diffusion or flow of the particles, and OCT uses coherence gating to collect light only scattered from a small volume for high-resolution structural imaging. Therefore, the integration of DLS and OCT enables high-resolution 3D imaging of diffusion and flow. We derived a theory under the assumption that static and moving particles are mixed within the OCT resolution volume and the moving particles can exhibit either diffusive or translational motion. Based on this theory, we developed a fitting algorithm to estimate dynamic parameters including the axial and transverse velocities and the diffusion coefficient. We validated DLS-OCT measurements of diffusion and flow through numerical simulations and phantom experiments. As an example application, we performed DLS-OCT imaging of the living animal brain, resulting in 3D maps of the absolute and axial velocities, the diffusion coefficient, and the coefficient of determination. (C) 2012 Optical Society of America