TISSUE DISTRIBUTION OF A COELOMIC-EPITHELIUM-RELATED ANTIGEN RECOGNIZED BY THE MONOCLONAL ANTIBODY-OC125
TISSUE DISTRIBUTION OF A COELOMIC-EPITHELIUM-RELATED ANTIGEN RECOGNIZED BY THE MONOCLONAL ANTIBODY-OC125
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DOI：
10.1097/00004347-198303000-00005
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发表时间：
1983-01-01
影响因子：
2.4
通讯作者：
COLVIN, RB
中科院分区：
文献类型：
--
作者：
KABAWAT, SE;BAST, RC;COLVIN, RB
OC125是一种鼠源性单抗，可识别与80%的浆液性、子宫内膜样癌、透明细胞瘤和未分化卵巢上皮性肿瘤相关的抗原决定簇(CA125)。本文用一种灵敏的生物素-亲和素免疫过氧化物酶技术来检测OC125与正常成人和胎儿组织以及非卵巢来源的肿瘤的反应性。在胎儿组织中，该抗体可与羊膜及体腔上皮的衍生物，即米氏上皮、腹膜、胸膜和心包的衬里细胞反应。在成体组织中，OC125与输卵管上皮、子宫内膜和子宫内膜呈阳性反应。在成人胸膜、心包和腹膜的间皮细胞上也检测到CA125决定簇，特别是在炎症和粘连区域。奇怪的是，正常胎儿和成人卵巢的表面上皮，被认为来自体腔上皮，除了包涵体囊、化生区和乳头状突起外，并不表达该决定簇。在非卵巢来源的肿瘤组织中，OC125仅与子宫内膜腺癌、子宫内膜癌、输卵管腺癌以及间皮瘤反应一致。在检测的非妇科肿瘤中，只有7例对OC125有反应。因此，OC125在胎儿和成人中检测到胎儿体腔上皮及其衍生物共有的分化抗原。显然，这种抗原在卵巢上皮形成过程中早期消失，并在某些反应性和肿瘤性病变中重新表达，这一过程可称为“分子化生”。
OC125, a murine monoclonal antibody, recognizes an antigenic determinant (CA125) that is associated with> 80% of epithelial ovarian neoplasms of serous, endometrioid, clear cell, and undifferentiated types. In the present report, a sensitive biotin-avidin immunoperoxidase technique was used to determine reactivity of OC125 with normal adult and fetal tissues, as well as with neoplasms of nonovarian origin. In fetal tissues, the antibody reacted with amnion and with derivatives of the coelomic epithelium, ie, the mül-lerian epithelium and the lining cells of the peritoneum, pleura, and pericardium. Among adult tissues, OC125 reacted with the epithelium of fallopian tubes, endometrium, and endocervix. The CA125 determinant was also detected on mesothelial cells in the adult pleura, pericardium, and peritoneum, particularly in areas of inflammation and adhesion. Curiously, the surface ep-ithelium of normal fetal and adult ovaries, thought to be derived from coelomic epithelium, did not express the determinant, except in inclusion cysts, areas of metaplasia, and papillary excrescences. Of neoplastic tissues of nonovarian origin, OC125 reacted consistently only with adenocarcinomas of the endocervix, endometrium, and fallopian tube, and with mesotheliomas. Only seven of 64 nongynecological tumors tested reacted with OC125. Thus, OC125 detects a differentiation antigen shared by fetal coelomic epithelium and its derivatives in the fetus and the adult. Apparently, this antigen disappears early in the course of formation of the ovarian epithelium and is reexpressed in certain reactive and neoplastic lesions, a process that could be termed “molecular metaplasia.”