QUANTITATIVE ASSESSMENT OF MYOCARDIAL COLLAGEN WITH PICROSIRIUS RED STAINING AND CIRCULARLY-POLARIZED LIGHT
QUANTITATIVE ASSESSMENT OF MYOCARDIAL COLLAGEN WITH PICROSIRIUS RED STAINING AND CIRCULARLY-POLARIZED LIGHT
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DOI：
10.1007/bf00788278
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发表时间：
1994-09-01
影响因子：
9.5
通讯作者：
PICKERING, JG
中科院分区：
文献类型：
--
作者：
WHITTAKER, P;KLONER, RA;PICKERING, JG
胶原蛋白在心脏的结构组织中起着重要的作用，因此，胶原纤维的直接可视化是心脏分析的重要组成部分。虽然线偏振光已被证明是在组织切片中检查心肌胶原蛋白的有效工具，但圆偏振光的使用可能提供优势和额外的可能性。我们用两种方法研究了使用圆偏振光对胶原分析的潜在增强作用。我们首先测量了心肌梗死后不同时间检查的瘢痕中胶原纤维的亮度，因此间接评估了双折射。其次，我们测量了心肌组织中胶原的含量，并将三色染色切片的Brightfield分析结果与天狼星红染色切片的圆偏振光分析结果进行了比较。我们观察到瘢痕中胶原纤维的最大亮度随着时间的推移而逐渐增加，胶原纤维亮度的相对分布随时间的变化而从低水平向高水平移动。我们发现三色染色的组织中胶原含量始终低于天狼星红染色的组织，并得出结论，三色染色低估了胶原的含量。这些研究提供的信息无法通过亮场分析获得，只能通过线偏振光分析获得部分信息。因此，使用圆偏振光的分析能够增强对组织的组织学评估，并可以提供对心肌胶原蛋白的组成和结构的额外见解。
Collagen plays a major role in the structural organization of the heart and therefore direct visualization of collagen fibers is a crucial component of cardiac analysis. Although linearly polarized light has proven an effective tool for the examination of myocardial collagen in histologic sections, the use of circularly polarized light may offer advantages and additional possibilities. We examined the potential enhancement of collagen analysis using circularly polarized light in two ways. We first measured the brightness, and hence indirectly assessed the birefringence, of collagen fibers in scars examined at different times after myocardial infarction. Secondly, we measured collagen content in myocardial tissue and compared results obtained from brightfield analysis of trichrome stained sections with those obtained from circularly polarized light analysis of picrosirius red stained sections. We observed a progressive increase in the maximum brightness of collagen fibers in the scar with time, and a time-dependent shift in the relative distribution of collagen fiber brightness from lower to higher levels. We found consistently lower values of collagen content in trichrome stained versus picrosirius red stained tissue, and concluded that trichrome staining underestimated collagen content. The information provided by these studies could not be obtained by brightfield analysis and could be only partially obtained from linearly polarized light analysis. Thus, analysis using circularly polarized light has the ability to enhance histologic assessment of tissue and can provide additional insights into the composition and structure of myocardial collagen.