Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli
Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli
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DOI：
10.1073/pnas.95.6.2885
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发表时间：
1998-03-17
影响因子：
11.1
通讯作者：
Lutkenhaus, J
中科院分区：
文献类型：
--
作者：
Mukherjee, A;Cao, CN;Lutkenhaus, J
细菌细胞分裂蛋白FtsZ组装成细胞动力学Z环，指导原核生物的胞质分裂。在大肠杆菌中，Z环的形成是通过诱导细胞分裂抑制因子Sula(SFIA)来防止的，SFIA是SOS反应的一个组成部分。在这里，我们证明了在体内保留这种抑制功能的雄性-秀拉融合在体外抑制了GTP酶的活性和FtsZ的聚合。雄性-SulA10在体内不阻断Z环的形成，在体外不能抑制GTP酶的活性和聚合。此外，在体内对Sula不敏感的FtsZ114不受雄性Sula的抑制。这些结果表明，苏拉通过阻断FtsZ聚合来阻止Z环的形成。
The bacterial cell division protein FtsZ assembles into the cytokinetic Z ring that directs cytokinesis in prokaryotes. In Escherichia coli the formation of the Z ring is prevented by induction of the cell division inhibitor SulA (SfiA), a component of the SOS response. Here we show that a MalE-SulA fusion that retains this inhibitory function in vivo inhibits the GTPase activity and polymerization of FtsZ in vitro. MalE-SulA10, which does not block Z ring formation in vivo, is unable to inhibit the GTPase activity and polymerization in vitro. Furthermore, FtsZ114 which is refractory to SulA in vivo, is not inhibited by MalE-SulA. These results indicate that SulA blocks Z ring formation by blocking FtsZ polymerization.