Formaldehyde kills spores of Bacillus subtilis by DNA damage and small, acid‐soluble spore proteins of the ααααααα/βββββββ‐type protect spores against this DNA damage
Formaldehyde kills spores of Bacillus subtilis by DNA damage and small, acid‐soluble spore proteins of the ααααααα/βββββββ‐type protect spores against this DNA damage
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甲醛通过 DNA 损伤杀死枯草芽孢杆菌的孢子，ααααααα/βββββββ 型的小型酸溶性孢子蛋白可保护孢子免受这种 DNA 损伤
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
P. Setlow
中科院分区：
文献类型：
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作者：
C. A. Loshon;P. Genest;B. Setlow;P. Setlow
用甲醛杀死枯草芽孢杆菌野生型孢子也会引起显着的诱变；缺乏两种主要 α/β 型小酸溶性孢子蛋白 (SASP) 的孢子（称为 α−β−）对甲醛杀灭和诱变更敏感。 recA 突变使野生型和 α−β− 孢子对甲醛处理敏感，当处理过的孢子萌发时，recA-lacZ 融合蛋白会显着表达。甲醛还会在野生型和 α−β− 孢子中引起蛋白质-DNA 交联。这些结果表明：(i) 甲醛至少部分通过 DNA 损伤杀死枯草芽孢杆菌孢子，(b) α/β 型 SASP 可能通过保护孢子 DNA 防止甲醛杀死孢子。
Killing of wild‐type spores of Bacillus subtilis with formaldehyde also caused significant mutagenesis; spores (termed α−β−) lacking the two major α/β‐type small, acid‐soluble spore proteins (SASP) were more sensitive to both formaldehyde killing and mutagenesis. A recA mutation sensitized both wild‐type and α−β− spores to formaldehyde treatment, which caused significant expression of a recA‐lacZ fusion when the treated spores germinated. Formaldehyde also caused protein–DNA cross‐linking in both wild‐type and α−β− spores. These results indicate that: (i) formaldehyde kills B. subtilis spores at least in part by DNA damage and (b) α/β‐type SASP protect against spore killing by formaldehyde, presumably by protecting spore DNA.
DOI：
10.1016/0027-5107(88)90122-4
发表时间：
1988-09-01
期刊：
MUTATION RESEARCH
影响因子：
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作者：
COSMA, GN;JAMASBI, R;MARCHOK, AC
通讯作者：
MARCHOK, AC