Evaluation of the in vivo genotoxicity of Allura Red AC (Food Red No. 40)
Evaluation of the in vivo genotoxicity of Allura Red AC (Food Red No. 40)
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DOI：
10.1016/j.fct.2015.09.007
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发表时间：
2015-10-01
影响因子：
4.3
通讯作者：
Honma, Masamitsu
中科院分区：
文献类型：
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作者：
Honma, Masamitsu
Allura Red AC（食品红 40 号）是一种红色偶氮染料，用于饮料和糖果产品中的食用色素。然而，其遗传毒性特性仍然存在争议。为了阐明体内生殖毒性，我们用 Allura Red AC 处理小鼠，并分别使用彗星试验、微核试验和转基因突变试验研究了 DNA 损伤（肝脏、腺胃）、染色体断裂/无源性（骨髓）和致突变性（肝脏、腺胃）的诱导。所有研究均按照经济合作与发展组织（OECD）的指导方针进行。尽管 Allura Red AC 的给药剂量达到了 OECD 指南建议的最大剂量，但在任何基因毒性终点均未观察到基因毒性效应。这些数据清楚地表明，没有证据表明在小鼠中给予最大剂量的 Allura Red AC 可能产生体内遗传毒性。 (C) 2015 Elsevier Ltd. 保留所有权利。
Allura Red AC (Food Red No. 40) is a red azo dye that is used for food coloring in beverage and confectionary products. However, its genotoxic properties remain controversial. To clarify the in vivo gentoxicity, we treated mice with Allura Red AC and investigated the induction of DNA damage (liver, glandular stomach), clastogenicity/anuegenicity (bone marrow), and mutagenicity (liver, glandular stomach) using Comet assays, micronucleus tests, and transgenic gene mutation assays, respectively. All studies were conducted in accordance with the Organization for Economic Co-operation and Development (OECD) guideline. Although Allura Red AC was administered up to the maximum doses recommended by the OECD guideline, no genotoxic effect was observed in any of the genotoxic endpoints. These data clearly show no evidence of in vivo genotoxic potential of Allura Red AC administered up to the maximum doses in mice. (C) 2015 Elsevier Ltd. All rights reserved.