Genotoxic and mutagenic assessment of iron oxide (maghemite-γ-Fe2O3) nanoparticle in the guppy Poecilia reticulata
Genotoxic and mutagenic assessment of iron oxide (maghemite-γ-Fe2O3) nanoparticle in the guppy Poecilia reticulata
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DOI：
10.1016/j.chemosphere.2017.05.061
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发表时间：
2017-09-01
期刊：
影响因子：
8.8
通讯作者：
Teixeira de Saboia-Morais, Simone Maria
中科院分区：
文献类型：
--
作者：
Qualhato, Gabriel;Rocha, Thiago Lopes;Teixeira de Saboia-Morais, Simone Maria
设计并应用于纳米修复过程中的纳米材料的环境风险引起了人们的关注，但其对水生生物的生态毒性效应尚不清楚。本研究合成了柠檬酸盐包被纳米粒(IONPs)，并研究了其对雌性孔雀鱼的遗传毒性和致突变作用。将鱼暴露于与环境有关的铁浓度(03 mg L-1)的IONPs中21天，并在实验开始时以及暴露后3、7、14和21天收集动物。通过DNA损伤(彗星试验)、微核试验(MN)和红细胞核异常(ENA)试验评价其遗传毒性和致突变性。结果表明，IONPs对网纹夜蛾的遗传毒性和致突变作用因暴露时间不同而不同。IONP在急性暴露(3d和7d)和长期暴露(14d和21d)后均可引起网纹拟青霉DNA损伤，但只有在长期暴露后才能观察到诱变效应。随着暴露时间的延长，DNA损伤和总ENA频率呈线性增加，表明长期暴露于IONPs后，网纹拟青霉红细胞具有较高的断裂和非优生诱发率。结果表明，网纹拟青霉红细胞是IONPs的生态毒性靶标。(C)2017爱思唯尔有限公司。保留所有权利。
The environmental risk of nanomaterials (NMs) designed and used in nanoremediation process is of emerging concern, but their ecotoxic effects to aquatic organism remains unclear. In this study, the citrate-coated (maghemite) nanoparticles (IONPs) were synthesized and its genotoxic and mutagenic effects were investigated in the female guppy Poecilia reticulate. Fish were exposed to IONPs at environmentally relevant iron concentration (03 mg L-1) during 21 days and the animals were collected at the beginning of the experiment and after 3, 7, 14 and 21 days of exposure. The genotoxicity and mutagenicity were evaluated in terms of DNA damage (comet assay), micronucleus (MN) test and erythrocyte nuclear abnormalities (ENA) frequency. Results showed differential genotoxic and mutagenic effects of IONPs in the P. reticulate according to exposure time. The IONP induced DNA damage in P. reticulata after acute (3 and 7 days) and long-term exposure (14 and 21 days), while the mutagenic effects were observed only after long-term exposure. The DNA damage and the total ENA frequency increase linearly over the exposure time, indicating a higher induction rate of clastogenic and aneugenic effects in P. reticulata erythrocytes after long-term exposure to IONPs. Results indicated that the P. reticulata erythrocytes are target of ecotoxicity of IONPs. (C) 2017 Elsevier Ltd. All rights reserved.