Erratum: Publisher Correction: Deconstructing and repurposing the light-regulated interplay between Arabidopsis phytochromes and interacting factors.
Erratum: Publisher Correction: Deconstructing and repurposing the light-regulated interplay between Arabidopsis phytochromes and interacting factors.
复制标题
勘误表：出版商更正：解构和重新利用拟南芥光敏色素和相互作用因子之间的光调节相互作用。
DOI：
10.1038/s42003-019-0726-6
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发表时间：
2019
影响因子：
5.9
通讯作者：
Möglich,Andreas
中科院分区：
文献类型：
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作者：
Golonka,David;Fischbach,Patrick;Jena,SiddharthaG;Kleeberg,JuliusRW;Essen,Lars-Oliver;Toettcher,JaredE;Zurbriggen,MatiasD;Möglich,Andreas
光敏色素光感受器介导植物对红光和远红光的适应性反应。这些反应通常需要光敏色素和其他蛋白质之间的光调节关联，其中包括光敏色素相互作用因子（PIF）。与拟南芥过敏色素B（AtPhy B）的相互作用定位于A的二分体AP B基序。thalianaPIFs（AtPIF）。为了解决缺乏定量相互作用的数据，我们构建和分析numerousAtPIF 3/6变异。红光激活的结合主要由APB N-末端介导，而C-末端调节结合并成为AtPIF 3和AtPIF 6的差异亲和力的基础。我们鉴定了尺寸缩小、单体或同二聚体状态的AtPIF变体，AtPhyB亲和力在10至700 nM之间。AtPIF变体在哺乳动物细胞中进行光遗传学部署，驱动光调节基因表达和膜募集，在某些情况下降低基础活性并增强调节反应。此外，我们的研究结果提供了迄今为止无法定量的洞察theAtPhyB：AtPIF相互作用的基础上重要的光依赖性反应在植物中。
Phytochrome photoreceptors mediate adaptive responses of plants to red and far-red light. These responses generally entail light-regulated association between phytochromes and other proteins, among them the phytochrome-interacting factors (PIF). The interaction withArabidopsis thalianaphytochrome B (AtPhyB) localizes to the bipartite APB motif of theA. thalianaPIFs (AtPIF). To address a dearth of quantitative interaction data, we construct and analyze numerousAtPIF3/6 variants. Red-light-activated binding is predominantly mediated by the APB N-terminus, whereas the C-terminus modulates binding and underlies the differential affinity ofAtPIF3 andAtPIF6. We identifyAtPIF variants of reduced size, monomeric or homodimeric state, and withAtPhyB affinities between 10 and 700 nM. Optogenetically deployed in mammalian cells, theAtPIF variants drive light-regulated gene expression and membrane recruitment, in certain cases reducing basal activity and enhancing regulatory response. Moreover, our results provide hitherto unavailable quantitative insight into theAtPhyB:AtPIF interaction underpinning vital light-dependent responses in plants.