Ceramic identity contributes to mechanical properties and osteoblast behavior on macroporous composite scaffolds.
Ceramic identity contributes to mechanical properties and osteoblast behavior on macroporous composite scaffolds.
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DOI：
10.3390/jfb3020382
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发表时间：
2012-05-23
影响因子：
4.8
通讯作者：
Leach JK
中科院分区：
文献类型：
--
作者：
Morales-Hernandez DG;Genetos DC;Working DM;Murphy KC;Leach JK
由金属、生物陶瓷或聚合物形成的植入物可提供自体移植物的替代物，用于治疗大的骨缺损。然而，对每种材料的限制促使对复合材料的检查，以利用单个组分的有益方面，并解决赋予聚合物基质生物活性行为的需要。我们假设陶瓷-聚合物复合材料中包含不同的生物陶瓷将改变植入物的物理性质和细胞成骨反应。为了测试这一点，制造由聚（丙交酯-共-乙交酯）（PLG）和羟基磷灰石（HA）、β-磷酸三钙（TCP）或生物活性玻璃（Bioglass 45 S ®，BG）形成的复合支架，并检查每个支架的物理性质。我们通过DNA含量定量细胞增殖，通过碱性磷酸酶（ALP）活性定量人成骨细胞（NHOsts）对复合支架的成骨反应，通过qPCR定量基因表达的变化。与BG-PLG支架相比，HA-PLG和TCP-PLG复合支架具有更大的压缩模量。21天后，BG-PLG基质上的NHOsts表现出比对照、HA-或TCP-PLG支架上的NHOsts更高的ALP活性，并且复合材料上的细胞在7天和21天之间表现出ALP活性的3倍增加，而对照支架上的ALP活性增加最小。与PLG对照上的细胞相比，复合支架上的NHOsts中的RUNX 2表达在第7天和第21天均较低，而BG-PLG支架上编码骨基质蛋白（COL 1A 1和COL 1A 2）的基因表达在两个时间点均较高。这些数据证明了在制造用于骨愈合的复合材料时选择陶瓷的重要性。
Implants formed of metals, bioceramics, or polymers may provide an alternative to autografts for treating large bone defects. However, limitations to each material motivate the examination of composites to capitalize on the beneficial aspects of individual components and to address the need for conferring bioactive behavior to the polymer matrix. We hypothesized that the inclusion of different bioceramics in a ceramic-polymer composite would alter the physical properties of the implant and the cellular osteogenic response. To test this, composite scaffolds formed from poly(lactide-co-glycolide) (PLG) and either hydroxyapatite (HA), β-tricalcium phosphate (TCP), or bioactive glass (Bioglass 45S®, BG) were fabricated, and the physical properties of each scaffold were examined. We quantified cell proliferation by DNA content, osteogenic response of human osteoblasts (NHOsts) to composite scaffolds by alkaline phosphatase (ALP) activity, and changes in gene expression by qPCR. Compared to BG-PLG scaffolds, HA-PLG and TCP-PLG composite scaffolds possessed greater compressive moduli. NHOsts on BG-PLG substrates exhibited higher ALP activity than those on control, HA-, or TCP-PLG scaffolds after 21 days, and cells on composites exhibited a 3-fold increase in ALP activity between 7 and 21 days versus a minimal increase on control scaffolds. Compared to cells on PLG controls, RUNX2 expression in NHOsts on composite scaffolds was lower at both 7 and 21 days, while expression of genes encoding for bone matrix proteins (COL1A1 and SPARC) was higher on BG-PLG scaffolds at both time points. These data demonstrate the importance of selecting a ceramic when fabricating composites applied for bone healing.