Immunocytochemical study of retinal diode laser photocoagulation in the rat
Immunocytochemical study of retinal diode laser photocoagulation in the rat
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DOI：
10.1136/bjo.80.12.1092
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发表时间：
1996-12-01
影响因子：
4.1
通讯作者：
McLeod, D
中科院分区：
文献类型：
--
作者：
Richardson, PRS;Boulton, ME;McLeod, D
目的-为了确定的性质的细胞浸润，细胞粘附分子的改变，和MHC II抗原表达在大鼠视网膜后二极管激光retinal photocoagulation.Method - 20正常李斯特大鼠进行了半导体激光光凝视网膜。在激光后0、1、5、13和33天摘除的眼睛的冷冻切片检查T细胞（R7.3）、CD 4 T细胞（W3/25）、活化的CD 4 T细胞（OX-40）、CD 8 T细胞（OX-8）、B细胞（OX-33）和巨噬细胞（OX-42）、MHC II抗原（OX-6）和E-选择素-1，VCAM-1，结果-视网膜半导体激光光凝刺激了外层视网膜和脉络膜的伤口愈合反应。在激光后13和33天，细胞浸润包括巨噬细胞和活化的CD 4 T细胞。内丛状层和内核层的胶质细胞仅在24小时表达MHC II抗原。ICAM-1抗原诱导的RPE细胞和Muller细胞在视网膜内层的激光后的所有时间间隔和强烈的染色ICAM-1存在于周围视网膜内迁移细胞在激光后13和33天。VCAM-1抗原表达诱导脉络膜血管内皮细胞和视网膜色素上皮细胞在13和33天后，激光是E-选择素-1抗原表达，这也是明显的局灶性在外部界膜与迁移的细胞相邻的burn.Conclusions -这些结果表明，细胞粘附分子的改变可能调节迁移和激活的视网膜色素上皮细胞，正常Lister大鼠视网膜的二极管激光光凝后外血-视网膜屏障和脉络膜处的巨噬细胞和CD 4 T细胞。
Aim - To determine the nature of the cellular infiltrate, alterations in cell adhesion molecules, and MHC II antigen expression in the rat retina following diode laser retinal photocoagulation.Method - 20 normal Lister rats underwent diode laser photocoagulation of the retina. Frozen sections ti om eyes enucleated at 0, 1, 5, 13, and 33 days post laser were examined for T cells (R7.3), CD4 T cells (W3/25), activated CD4 T cells (OX-40), CD8 T cells (OX-8), B cells (OX-33), and macrophages (OX-42), MHC II antigen (OX-6), and E-Selectin-l, VCAM-1, and ICAM-1.Results - Retinal diode laser photocoagulation stimulated a wound healing response in the outer retina and choroid. The cellular infiltrate included macrophages and activated CD4 T cells at 13 and 33 days post laser. Glial cells in the inner plexiform and inner nuclear layers expressed MHC II antigen at 24 hours only. ICAM-1 antigen was induced in RPE cells and in Muller cells in the inner retina at all time intervals post laser and intense staining for ICAM-1 was present around intraretinal migrated cells at 13 and 33 days post laser. VCAM-1 antigen expression was induced in the choroidal vascular endothelium and RPE at 13 and 33 days after laser as was E-Selectin-l antigen expression which was also evident focally at the external Limiting membrane in association with migrated cells adjacent to the burn.Conclusions - These results suggest that alterations in cell adhesion molecules may regulate the migration and activation of retinal pigment epithelium, macrophages and CD4 T cells at the outer blood-retinal barrier and choroid following diode laser photocoagulation of the normal Lister rat retina.