CYTOTOXIN-CONVERTING PHAGES, PHI-CTX AND PS21, ARE R-PYOCIN-RELATED PHAGES
CYTOTOXIN-CONVERTING PHAGES, PHI-CTX AND PS21, ARE R-PYOCIN-RELATED PHAGES
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DOI：
10.1016/0378-1097(94)00329-7
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发表时间：
1994-10-01
影响因子：
2.1
通讯作者：
TERAWAKI, Y
中科院分区：
文献类型：
--
作者：
HAYASHI, T;MATSUMOTO, H;TERAWAKI, Y
phi CTX 是铜绿假单胞菌的温带噬菌体，带有编码细胞毒素 (CTX) 的 ctx 基因。我们通过血清学和分子分析将 phi CTX 鉴定为 R 化脓菌素相关噬菌体，基于这样的发现：噬菌体的感染性被针对 R 化脓菌素和 R 化脓菌素相关噬菌体的抗血清抑制，并且 phi CTX 基因组显示出与 PS17（R 化脓菌素相关噬菌体的代表）基因组以及 脓毒素 R2 基因。另一种新的 CTX 转化、R 脓菌素相关噬菌体，名为 PS21，是从铜绿假单胞菌产生 CTX 的菌株中分离出来的，表明 ctx 基因由 R 脓菌素相关噬菌体家族的某些成员分布。
phi CTX is a temperate phage of Pseudomonas aeruginosa harbouring the ctx gene that encodes cytotoxin (CTX). We identified phi CTX as an R pyocin-related phage, by serological and molecular analysis, based on the findings that the infectivity of the phage was inhibited with the antisera directed R pyocins and R pyocin-related phages and that the phi CTX genome showed DNA homology to the genome of PS17 (a representative of the R pyocin-related phages) as well as to the pyocin R2 genes. Another new CTX-converting, R pyocin-related phage named PS21 was isolated from a CTX-producing strain of P. aeruginosa, suggesting the distribution of the ctx gene by certain members of R pyocin-related phage family.