Multifunctional single-layered graphene quantum dots used for diagnosis of mitochondrial malfunction-related diseases
Multifunctional single-layered graphene quantum dots used for diagnosis of mitochondrial malfunction-related diseases
复制标题
多功能单层石墨烯量子点用于诊断线粒体功能障碍相关疾病
DOI：
10.1021/acsbiomaterials.9b01395
复制
发表时间：
2020
影响因子：
--
通讯作者：
Xiao Yong Tong
中科院分区：
文献类型：
--
作者：
Xiao Li Jiang;Jia Hui Liu;Yan Ting Que;Yu Mei Que;Ping Ping Hu;Cheng Zhi Huang;Xiao Yong Tong
线粒体是真核生物中的重要细胞器，可以有效地产生用于各种生物活性的5 '-三磷酸腺苷（ATP），ATP合成过程中的任何缺陷都可能导致线粒体功能障碍，并直接与多种医学疾病有关。监测细胞中的ATP变化是线粒体疾病早期诊断的关键。本文构建了具有明亮绿色发射的多功能单层石墨烯量子点（s-GQDs），其表现出对ATP的强结合亲和力和良好的线粒体靶向能力。使用所提出的s-GQD，我们成功地区分了从转基因小鼠分离的原代平滑肌细胞（杂合子肌浆网/内质网Ca 2 + ATP酶（SERCA）2 C674 S敲入小鼠）或其同窝对照，表明s-GQD是研究细胞代谢和线粒体功能障碍相关疾病的有前景的探针，靶向内质网应激是调节与SERCA 2失活线粒体功能障碍相关的代谢途径的有效途径。
Mitochondria are critical organelles in eukaryotes that efficiently generate adenosine 5′-triphosphate (ATP) for various biological activities, and any defect in the process of ATP synthesis may lead to mitochondrial dysfunction and directly link to a variety of medical disorders. Monitoring the ATP variations in cells is key for innovative early diagnosis of mitochondrial diseases. Herein, multifunctional single-layered graphene quantum dots (s-GQDs) with bright green emission were constructed, which exhibit strong binding affinity for ATP and good mitochondria targeting ability. Using the proposed s-GQDs, we successfully discriminated the primary smooth muscle cells isolated from the transgenic mouse (heterozygote sarco/endoplasmic reticulum Ca2+ATPase (SERCA) 2 C674S knock-in mouse) with mitochondrial disorders or their littermate controls, indicating s-GQDs as promising probes for the study of cell metabolism and mitochondrial malfunction-related diseases, and targeting endoplasmic reticulum stress is an effective way to modulate metabolic pathways relevant to SERCA 2 inactivity mitochondrial dysfunction.