Enhanced production of leukotrienes by peripheral leukocytes and specific IgE antibodies in patients with chronic obstructive pulmonary disease.
Enhanced production of leukotrienes by peripheral leukocytes and specific IgE antibodies in patients with chronic obstructive pulmonary disease.
复制标题
慢性阻塞性肺病患者外周白细胞和特异性 IgE 抗体产生的白三烯增强。
DOI：
10.1067/mai.2001.112694
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Y. Tanizaki
中科院分区：
文献类型：
--
作者：
F. Mitsunobu;T. Mifune;Y. Hosaki;K. Ashida;H. Tsugeno;M. Okamoto;S. Takata;Y. Tanizaki
背景
白三烯（LT）和IgE介导的过敏反应如何反映慢性阻塞性肺疾病（COPD）患者的临床特征仍不清楚。
目的
我们的目标是确定LTB 4和LTC 4是否与气道阻塞相关，以及IgE介导的过敏是否会影响COPD患者LT和支气管高反应性的产生。
方法
我们测量了肺功能，乙酰甲胆碱支气管高反应性，并产生LTB 4和LTC 4从周围白细胞刺激钙离子载体A23187的存在下，对吸入性过敏原的特异性IgE抗体。
结果
COPD患者的白细胞产生的LTB 4（有过敏反应，P <0.001;无过敏反应，P <0.001）和LTC 4（有过敏反应，P <0.001;无过敏反应，P <0.01）显著高于对照组的白细胞。过敏性COPD患者的LTC 4生成量显著高于非过敏性COPD患者（P <0.01），但LTB 4生成量无显著差异。COPD患者FEV（1）与LTB 4（有过敏反应，r =-0.556，P =.0375;无过敏反应，r =-0.731，P =.0046）和LTC 4（有过敏反应，r =-0.764，P =.0043;无过敏反应，r =-0.526，P =.0414）水平显著相关。无过敏反应的COPD患者最小乙酰甲胆碱剂量的log（10）显著高于有过敏反应的患者（P <0.05）。
结论
在COPD患者中观察到外周血白细胞产生的LT增强，并且针对吸入性变应原的特异性IgE抗体的存在增强了LTC 4的产生、支气管高反应性以及LTC 4产生与气道阻塞之间的关系。
BACKGROUND
How leukotrienes (LTs) and IgE-mediated allergy reflect clinical features in patients with chronic obstructive pulmonary disease (COPD) remains unclear.
OBJECTIVE
Our goal was to determine whether LTB4 and LTC4 would correlate with airway obstruction and whether IgE-mediated allergy would influence the generation of LTs and bronchial hyperresponsiveness in patients with COPD.
METHODS
We measured the pulmonary function, methacholine bronchial hyperresponsiveness, and generation of LTB4 and LTC4 from peripheral leukocytes stimulated with calcium ionophore A23187 in relation to the presence of specific IgE antibodies against inhalant allergens.
RESULTS
The leukocytes of patients with COPD generated significantly more LTB4 (with allergy, P <.001; without allergy, P <.001) and LTC4 (with allergy, P <.001; without allergy, P <.01) than the leukocytes of the control subjects. LTC4 production was significantly higher in the allergic COPD subjects than in the nonallergic COPD patients (P <.01), but the amount of LTB4 generated was not significantly different. FEV(1) significantly correlated with the level of both LTB4 (with allergy, r = -0.556, P =.0375; without allergy, r = -0.731, P =.0046) and LTC4 (with allergy, r = -0.764, P =.0043; without allergy, r = -0.526, P =.0414) generation in COPD. The log(10) of the minimum dose of methacholine was significantly higher in COPD patients without allergy than in those with allergy (P <.05).
CONCLUSION
Enhanced LT generation from peripheral leukocytes is observed in patients with COPD, and the presence of specific IgE antibodies against inhalant allergens enhances LTC4 generation, bronchial hyperresponsiveness, and the relationship between LTC4 generation and airway obstruction.
影响因子：
4.4
作者：
W. Owen;R. Soberman;T. Yoshimoto;A. Sheffer;R. Lewis;K. Austen
通讯作者：
W. Owen;R. Soberman;T. Yoshimoto;A. Sheffer;R. Lewis;K. Austen