PC12 live cell ultrasensitive neurotransmitter signaling using high quantum yield sulphur doped carbon dots and its extracellular Ca2+ ion dependence
PC12 live cell ultrasensitive neurotransmitter signaling using high quantum yield sulphur doped carbon dots and its extracellular Ca2+ ion dependence
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DOI：
10.1016/j.snb.2017.01.145
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发表时间：
2017-06-01
影响因子：
8.4
通讯作者：
Nandi, Chayan Kanti
中科院分区：
文献类型：
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作者：
Gupta, Abhishek;Nandi, Chayan Kanti
合成明亮、光稳定且无毒的掺杂荧光材料始终是其成功应用的巨大挑战。另一方面，由于其浓度非常低，低至nM水平，活细胞内神经递质的检测也非常具有挑战性。在本报告中，报道了一种新颖的一步合成方法，用于合成高亮多色荧光硫掺杂碳点（CND），这是一种新型碳纳米材料，量子产率（QY）高达58％。合成的 CND 对神经递质多巴胺 (DA) 检测显示出超灵敏传感，无需任何表面功能化，使用荧光测定法，水中浓度低至 47 pM，血浆中浓度低至 92 pM。有趣的是，即使存在高浓度的其他相关干扰分子，CND 对 DA 也表现出非常高的选择性。 CND 在缺氧环境下非常有效地检测活 PC12 细胞中的 DA。我们进一步提供了关于神经元细胞内 Ca2+ 依赖性 DA 释放的丰富信息。细胞外Ca2+是否加速突触小泡的DA释放还是储存的细胞内Ca2+足以释放DA仍然是一个有争议的问题。我们的数据表明，神经元细胞内的细胞外 Ca2+ 流入确实加速了 DA 的释放。 (C) 2017 Elsevier B.V. 保留所有权利。
Synthesizing bright, photostable and non-toxic doped fluorescent material is always a great challenge for its successful application. On the other hand, because of its very low concentration down to nM level, detection of neurotransmitter inside live cell is also very challenging. In this report, a novel one-step synthetic method for the synthesis of highly bright multicolor fluorescent sulphur doped carbon dots (CNDs), a new class of carbogenic nanomaterials, with quantum yield (QY) upto 58% is reported. The synthesized CNDs show ultrasensitive sensing for neurotransmitter dopamine (DA) detection without any surface functionalization using fluorometric assay down to 47 pM in water and 92 pM in blood plasma. Interestingly, the CNDs show very high selectivity to DA even in presence of high concentrations of other relevant interfering molecules. The CNDs are very efficient to detect the DA in live PC12 cell under hypoxic environment. We further provide fruitful information on Ca2+ dependent DA release inside neuron cells. It is still a debatable problem whether extracellular Ca2+ accelerates the DA release from synaptic vesicles or stored intracellular Ca2+ is sufficient for DA release. Our data shows that indeed extracellular Ca2+ influx inside neuron cells accelerates the DA release. (C) 2017 Elsevier B.V. All rights reserved.