Down-regulation of OsSPX1 causes high sensitivity to cold and oxidative stresses in rice seedlings.
Down-regulation of OsSPX1 causes high sensitivity to cold and oxidative stresses in rice seedlings.
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DOI：
10.1371/journal.pone.0081849
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Su Z
中科院分区：
文献类型：
--
作者：
Wang C;Wei Q;Zhang K;Wang L;Liu F;Zhao L;Tan Y;Di C;Yan H;Yu J;Sun C;Chen WJ;Xu W;Su Z
水稻SPX结构域基因OsSPX 1在磷酸盐（Pi）信号网络中起着重要作用。我们之前的工作表明，OsSPX 1在烟草和拟南芥中的组成型过表达提高了植物的耐冷性，同时也降低了叶片总Pi。在本研究中，我们获得了OsSPX 1反义和正义转基因水稻株系，发现OsSPX 1的下调导致水稻幼苗对低温和氧化胁迫的高度敏感性。与野生型和OsSPXl正义转基因株系相比，对照、冷处理和甲基紫精（MV）处理的OsSPXl反义转基因株系的幼苗叶片中积累了更多的过氧化氢。谷胱甘肽作为活性氧清除剂可以保护反义转基因株系免受低温和MV胁迫。水稻全基因组基因芯片分析结果表明，OsSPX 1反义基因显著下调了部分氧化胁迫标记基因（如谷胱甘肽S-转移酶和P450 s）和Pi信号通路相关基因（如OsPHO 2）。芯片结果经实时荧光定量RT-PCR验证。研究表明，OsSPX 1可能参与了水稻幼苗叶片氧化胁迫、低温胁迫和磷稳态之间的交互作用。
Rice SPX domain gene, OsSPX1, plays an important role in the phosphate (Pi) signaling network. Our previous work showed that constitutive overexpression of OsSPX1 in tobacco and Arabidopsis plants improved cold tolerance while also decreasing total leaf Pi. In the present study, we generated rice antisense and sense transgenic lines of OsSPX1 and found that down-regulation of OsSPX1 caused high sensitivity to cold and oxidative stresses in rice seedlings. Compared to wild-type and OsSPX1-sense transgenic lines, more hydrogen peroxide accumulated in seedling leaves of OsSPX1-antisense transgenic lines for controls, cold and methyl viologen (MV) treatments. Glutathione as a ROS scavenger could protect the antisense transgenic lines from cold and MV stress. Rice whole genome GeneChip analysis showed that some oxidative-stress marker genes (e.g. glutathione S-transferase and P450s) and Pi-signaling pathway related genes (e.g. OsPHO2) were significantly down-regulated by the antisense of OsSPX1. The microarray results were validated by real-time RT-PCR. Our study indicated that OsSPX1 may be involved in cross-talks between oxidative stress, cold stress and phosphate homeostasis in rice seedling leaves.
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