Marinomonas mediterranea is a lysogenic bacterium that synthesizes R-bodies
Marinomonas mediterranea is a lysogenic bacterium that synthesizes R-bodies
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DOI：
10.1099/mic.0.26524-0
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发表时间：
2003-09-01
期刊：
影响因子：
2.8
通讯作者：
Sanchez-Amat, A
中科院分区：
文献类型：
--
作者：
Hernández-Romero, D;Lucas-Elío, P;Sanchez-Amat, A
透射电子显微镜显示，产生黑色素的海洋细菌Marinomonas Mediterranea能合成R-小体。这些结构以前在草履虫和一些自由生活细菌的一些专性共生体中被描述过，这些共生体都不是从海水中分离出来的。在其他微器官系统中，R小体的合成与染色体外元素有关。相应地，用丝裂霉素C或紫外线辐射诱导的稻瘟病菌产生了类似细菌素的缺陷噬菌体，表明它是一种溶原菌。已分离到两株原噬菌体复制缺陷的丝裂霉素C抗性菌株。这些突变株和先前获得的菌株NGc1、T102和T103，后者在编码组氨酸激酶传感器的PPOS基因上突变，不仅在噬菌体复制方面受到影响，而且在多酚氧化酶活性和黑色素合成方面也受到影响，这表明所有这些过程之间存在控制关系。
The melanogenic marine bacterium Marinomonas mediterranea synthesizes R-bodies as revealed by transmission electron microscopy. These structures were previously described in some obligate symbionts of paramecia and some free-living bacteria, none of which was isolated from sea water. In other micro-organ isms, the synthesis of R-bodies has been related to extrachromosomal elements. Accordingly, M. mediterranea induction by mitomycin C or UV radiation resulted in the production of defective phages resembling bacteriocins, indicating that it is a lysogenic bacterium. Two mitomycin-C-resistant strains defective in prophage replication have been isolated. These mutants, and the previously obtained strains ngC1, T102 and T103, the latter mutated in the ppoS gene encoding a sensor histidine kinase, are affected not only in phage replication but also in polyphenol oxidase activities and melanin synthesis, suggesting a relationship between the control of all these processes.